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首页> 外文期刊>The Journal of Experimental Biology >The major vault protein is related to the toxic anion resistance protein (TelA) family
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The major vault protein is related to the toxic anion resistance protein (TelA) family

机译：主要的穹顶蛋白与毒性阴离子抗性蛋白（TelA）家族有关

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摘要

Vaults are barrel-shaped ribonucleoprotein particles that are abundant in certain tumors and multidrug resistant cancer cells. Prokaryotic relatives of the major vault protein, MVP, have not been identified. We used sequence analysis and molecular modeling to show that MVP and the toxic anion resistance protein, TelA of Rhodobacter sphaeroides strain 2.4.1, share a novel fold that consists of a three-stranded antiparallel beta-sheet. Because of this strong structural correspondence, we examined whether mammalian cell vaults respond to tellurite treatment. In the presence of the oxyanion tellurite, large vault aggregates, or vaultosomes, appear at the cell periphery in 15 min or less. Vaultosome formation is temperature-dependent, reversible, and occurs in normal human umbilical vein endothelial cells as well as transformed HeLa cervical cancer cells. Vaultosome formation is not restricted to tellurite and occurs in the presence of other toxic oxyanions (selenate, selinite, arsenate, arsenite, vanadate). In addition, vaultosomes form independently from other stress-induced ribonucleoprotein complexes, stress granules and aggresomes. Vaultosome formation is therefore a unique cellular response to an environmental toxin.
机译：穹顶是桶形核糖核蛋白颗粒，在某些肿瘤和多药耐药性癌细胞中含量很高。主要穹顶蛋白MVP的原核亲属尚未确定。我们使用序列分析和分子建模来显示MVP和球形抗红球菌菌株2.4.1的毒性阴离子抗性蛋白TelA，具有由三链反平行β-折叠构成的新折叠。由于这种强烈的结构对应关系，我们检查了哺乳动物细胞穹顶是否对亚碲酸盐处理有反应。在含氧阴离子亚碲酸盐的存在下，在15分钟或更短的时间内，大的穹顶聚集体或穹顶体就会出现在细胞周围。穹顶小体形成是温度依赖性的，可逆的，并发生在正常人脐静脉内皮细胞以及转化的HeLa宫颈癌细胞中。穹o体的形成不仅仅限于亚碲酸盐，而且还存在于其他有毒的含氧阴离子（硒酸根，亚硒酸根，砷酸根，砷酸根，钒酸根）下。此外，穹顶小体独立于其他应激诱导的核糖核蛋白复合物，应激颗粒和聚集体形成。因此，穹顶小体形成是对环境毒素的独特细胞应答。

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