• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Experimental Biology >Ovary ecdysteroidogenic hormone activates egg maturation in the mosquito Georgecraigius atropalpus after adult eclosion or a blood meal
【24h】

Ovary ecdysteroidogenic hormone activates egg maturation in the mosquito Georgecraigius atropalpus after adult eclosion or a blood meal

机译：卵巢蜕皮类固醇激素可在成年鸡舍或放血后激活Georgecraigius atropalpus蚊子的卵成熟

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The rockpool mosquito, Georgecraigius atropalpus, is a facultatively autogenous species that produces its first egg clutch without a blood meal shortly after emergence. Several days after depositing this clutch, females must take a blood meal to produce a second egg clutch. Decapitation of females shortly after emergence or blood ingestion prevents egg maturation. Here, we report that a single injected dose of the neuropeptide ovary ecdysteroidogenic hormone (OEH) fully restored egg maturation in decapitated females in both circumstances. This neuropeptide and two insulin-like peptides (ILPs) are potent gonadotropins in the related yellow fever mosquito, Aedes aegypti. ILP3 was marginally restorative in decapitated G. atropalpus, and ILP4 had no effect. Egg maturation in non- and blood-fed G. atropalpus was dependent on the enzymatic mobilization of amino acids from stored protein or the blood meal for yolk protein (vitellogenin, VG) synthesis and uptake by oocytes. We further show that OEH stimulates serine protease activity in the fat body of newly eclosed females or in the midgut of blood-fed ones, and ecdysteroid hormone production by the ovaries of both females. In contrast, only 20-hydroxyecdysone stimulated VG synthesis in the fat body of non- and blood-fed females. Using RNA interference to knock down expression of the insulin receptor, we found that OEH still fully restored autogenous egg maturation. In summary, our results identify OEH as a primary regulator of egg maturation in both autogenous and blood-fed G. atropalpus females and suggest the shift from blood meal-dependent to blood meal-independent release of OEH is a key factor in the evolution of autogeny in this species.
机译：岩塘蚊Georgecraigius atropalpus是一种具有自生能力的物种，在出苗后不久就产生了第一个不带血粉的卵子。放置离合器后的几天，雌性必须吃血粉以产生第二个卵离合器。出现或摄入血液后不久将雌性斩首可防止卵成熟。在这里，我们报告说，在两种情况下，单次注射剂量的神经肽卵巢蜕皮类固醇激素（OEH）都能完全恢复断头女性的卵成熟。这种神经肽和两种胰岛素样肽（ILP）在相关的黄热病蚊埃及伊蚊中是有效的促性腺激素。 ILP3在断头的G. atropalpus中几乎不能恢复，而ILP4没有作用。非饲喂和带血的阿托巴链霉菌的卵成熟取决于卵黄蛋白（玻璃体生成素，VG）的合成和卵母细胞摄取的氨基酸的酶促动。我们进一步表明，OEH刺激新近封闭的雌性的脂肪体内或血源性雌性的中肠中的丝氨酸蛋白酶活性，以及​​雌性卵巢的蜕皮甾类激素产生。相比之下，只有20-羟基蜕皮酮能刺激非和有血缘的女性脂肪体内的VG合成。使用RNA干扰敲低胰岛素受体的表达，我们发现OEH仍能完全恢复自体卵的成熟。总而言之，我们的研究结果表明，OEH是自体和血源性阿托拉喀斯女性的卵成熟的主要调节剂，并表明OEH从血粉依赖性释放到非血粉依赖性释放是OEH进化的关键因素。该物种的自体。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号