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首页> 外文期刊>The Journal of Experimental Biology >The effects of cell ageing on protein synthesis in rainbow trout (Oncorhynchus mykiss) red blood cells
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The effects of cell ageing on protein synthesis in rainbow trout (Oncorhynchus mykiss) red blood cells

机译：细胞老化对虹鳟（Oncorhynchus mykiss）红细胞蛋白质合成的影响

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摘要

The effects of cell age on protein synthesis were examined ill the nucleated red blood cells of rainbow trout (Oncorhynchus mykiss). Total DNA content was unaffected by cell age, whereas total PNA content in young red blood cells was roughly ten times as high as that in old red blood cells. The mRNA levels for haemoglobin, carbonic anhydrase and the chloride/bicarbonate (Cl-IHCO3-) exchanger were also approximately tenfold higher in young red blood cells. Although young red blood cells synthesized roughly five times more protein under steady-state conditions, total protein concentration was not affected by cell age. Despite large reductions in mRNA levels with red blood cell ageing, the concentrations and/or activities of the respiratory proteins were largely preserved. In contrast, the ability to mount a heat shock response was greatly reduced in older red blood cells, Young red blood cells produced 13 times more heat shock protein 70 mRNA following heat shock and four times more 70 kDa protein after recovery. They also transcribed much more heat shock cognate 71 and heat shock factor mRNA than did older red blood cells under steady-state conditions. [References: 50]
机译：在虹鳟鱼（Oncorhynchus mykiss）的有核红细胞中检查了细胞年龄对蛋白质合成的影响。总DNA含量不受细胞年龄的影响，而年轻红细胞中总PNA含量大约是旧红细胞中总PNA含量的十倍。在年轻的红细胞中，血红蛋白，碳酸酐酶和氯化物/碳酸氢盐（Cl-IHCO3-）交换子的mRNA水平也大约高十倍。尽管年轻的红细胞在稳态条件下合成的蛋白质大约多五倍，但总蛋白质浓度不受细胞年龄的影响。尽管随着红细胞衰老，mRNA水平大大降低，但呼吸蛋白的浓度和/或活性仍得以保留。相反，在老年红细胞中，引起热激反应的能力大大降低。年轻红细胞在热激后产生的热激蛋白70 mRNA的含量高出13倍，在恢复后产生的70 kDa蛋白的含量高出四倍。他们还转录了比稳态条件下的老龄红细胞更多的热休克同源71和热休克因子mRNA。 [参考：50]

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