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首页> 外文期刊>The Journal of Experimental Biology >Two myostatin genes are differentially expressed in myotomal muscles ofthe trout (Oncorhynchus mykiss)
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Two myostatin genes are differentially expressed in myotomal muscles ofthe trout (Oncorhynchus mykiss)

机译：两个肌肉生长抑制素基因在鳟鱼的肌肌中差异表达（Oncorhynchus mykiss）

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摘要

Myostatin (GDF8) has been shown to be a major genetic determinant of skeletal muscle growth in mammals. In this study, we report the cloning of two trout cDNAs that encode two distinct myostatin-related proteins. The presence in this fish species of two myostatin genes (Tmyostatin 1 and Tmyostatin 2) probably results from the recent tetraploidisation of the salmonid genome. A comparative reverse-transcriptase-linked polymerase chain reaction assay revealed that Tmyostatin 1 mRNA was present ubiquitously in trout tissues, while Tmyostatin 2 mRNA expression was restricted to muscle and brain. In developing muscle, Tmyostatin I expression was observed in eyed-stage embryos well before hatching, whereas Tmyostatin 2 was expressed only in free-swimming larvae. In myotomal muscle from adult animals, Tmyostatin I mRNA accumulation was similar in both slow- and fast-twitch fibres, and its concentration did not change during the muscle wasting associated with sexual maturation. In contrast, Tmyostatin 2 mRNA accumulated predominantly in slow-twitch fibres, and its concentration decreased dramatically in wasting muscles from maturing animals. This work shows that two distinct myostatin genes are present in the trout genome. Furthermore, it indicates that these two trout myostatin genes (I) exhibit a distinct expression pattern in muscle and non-muscle tissues and (ii) are not upregulated during the muscle wasting that accompanies sexual maturation.
机译：肌生长抑制素（GDF8）已被证明是哺乳动物骨骼肌生长的主要遗传决定因素。在这项研究中，我们报告了两个鳟鱼cDNA的克隆，它们编码两种不同的肌肉生长抑制素相关蛋白。这种鱼类中存在两个肌生长抑制素基因（肌生长抑制素1和肌生长抑制素2）可能是由于鲑鱼基因组最近的四倍体化所致。对比的逆转录酶连接的聚合酶链反应分析表明，鳟鱼组织中普遍存在肌凝抑素1 mRNA，而肌凝抑素2 mRNA的表达仅限于肌肉和大脑。在发育中的肌肉中，在孵化前很早就在眼期胚胎中观察到了肌生成抑制素I表达，而肌生成抑制素2仅在自由游动的幼虫中表达。在成年动物的肌肌中，肌腱抑制素I mRNA的积累在慢速和快肌中都相似，并且在与性成熟相关的肌肉消瘦过程中其浓度没有变化。相反，Tmyostatin 2 mRNA主要在缓慢抽动的纤维中积累，而在成熟动物的肌肉消瘦中其浓度急剧下降。这项工作表明鳟鱼基因组中存在两个不同的肌肉生长抑制素基因。此外，这表明这两个鳟鱼肌肉生长抑制素基因（I）在肌肉和非肌肉组织中表现出不同的表达模式，并且（ii）在伴随性成熟的肌肉消瘦过程中并未上调。

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