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首页> 外文期刊>The Southeast Asian journal of tropical medicine and public health >Comparison of Vi serology and nested PCR in diagnosis of chronic typhoid carriers in two different study populations in typhoid endemic area of India.
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Comparison of Vi serology and nested PCR in diagnosis of chronic typhoid carriers in two different study populations in typhoid endemic area of India.

机译：病毒学和巢式PCR在印度伤寒流行地区两个不同研究人群中诊断慢性伤寒携带者的比较。

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摘要

For detection of chronic typhoid carriers, nested PCR targeting flagellin the gene of Salmonella enterica subspecies enterica serotype Typhi was carried out on DNA extracted from hepatobiliary specimens from 424 autopsies which were apparently free from gallbladder pathology on postmortem examination. The second study population was 508 healthy volunteers, who did not suffer from typhoid fever during the preceding year and whose sera were subjected to detection of carriage by estimation of Vi antibody levels using an indirect hemagglutination assay. Males of both study populations had comparable rates of detection by the two methods, 6.3% by PCR and 4.1% by Vi serology. Similarly, females in both study groups had comparable frequency of detection of chronic typhoid carriage using the two methods, ie 13.1% by PCR and 15.1% by Vi serology. S. Typhi specific immunosuppression could be speculated in females of 51-60 years as only 40% were positive by Vi serology against 100% by nested PCR. Vi serology may be recommended for community based detection of chronic typhoid carriers.
机译：为了检测慢性伤寒携带者，对从424例尸检的肝胆标本中提取的DNA进行了以鞭毛蛋白为肠沙门氏菌肠型血清型Typhi基因的巢式PCR，在死后检查中显然没有胆囊病理。第二个研究人群是508名健康志愿者，他们在上一年未患伤寒，其血清通过间接血凝测定法评估Vi抗体水平而进行了运输检测。两个研究人群的男性在两种方法中的检出率相当，PCR检出率为6.3％，病毒血清学检出率为4.1％。同样，两个研究组中的女性使用两种方法检测慢性伤寒菌的频率相当，即PCR检出率为13.1％，病毒血清学检出率为15.1％。可以推测，在51-60岁的女性中，伤寒沙门氏菌具有特异性的免疫抑制作用，因为Vi血清学检测到的阳性率为40％，巢式PCR检测的阳性率为100％。血清学可能被推荐用于慢性伤寒携带者的社区检测。

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