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首页> 美国卫生研究院文献>Journal of Clinical and Diagnostic Research : JCDR >Mix-infection of S. Typhi and ParaTyphi A in Typhoid Fever and Chronic Typhoid Carriers: A Nested PCR Based Study in North India
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Mix-infection of S. Typhi and ParaTyphi A in Typhoid Fever and Chronic Typhoid Carriers: A Nested PCR Based Study in North India

机译：伤寒和慢性伤寒携带者中伤寒沙门氏菌和副伤寒甲的混合感染：基于巢式PCR的北印度研究

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>Introduction: Enteric fever is a systemic disease caused by Salmonella organism such as serotypes Typhi and ParaTyphi A, B, C. Salmonella ParaTyphi A contributes more than 50% of all the enteric fever cases and it has recently been projected as an emerging pathogen.>Materials and Methods: The present study was aimed to detect Salmonella Typhi and ParaTyphi A in urine, blood and stool specimens collected from cases of enteric fever (110), chronic typhoid carriers (46) and healthy controls (75) to explore the possibility of mixed infection by nested PCR. A new nested PCR primer was designed targeting putative fimbrial protein (stkG) gene which is one of the fimbrial gene families to Salmonella ParaTyphi A and for S. Typhi already reported primers targeting flagellin (fliC) gene.>Results: Large volume of urine specimens (15 ml) was found to be the best for detection of Salmonella serotypes. The urine sample was found to have mixed-infection by both the serotypes in 40.9% of the cases but lower in blood (27.3%) and stool (13.6%).>Conclusion: The present study concludes that occurrence of mixed infection may be quite frequent in typhoid and chronic typhoid carriers’ individuals, although the reported recent rise in ParaTyphi A incidence may not be real.
机译：>简介：肠热是由沙门氏菌引起的一种全身性疾病，例如血清型Typhi和ParaTyphi A，B，C。沙门氏菌ParaTyphi A占所有肠热病例的50％以上，最近>材料和方法：本研究旨在检测从肠热（110），慢性伤寒携带者（110）中收集的尿液，血液和粪便标本中的鼠伤寒沙门氏菌和副伤寒杆菌A。 46）和健康对照组（75），探讨通过巢式PCR混合感染的可能性。设计了一种针对推定的纤维蛋白（stkG）基因的新型巢式PCR引物，该基因是ParaTyphi沙门氏菌A和S. Typhi的纤维基因家族之一。Typhi已经报道了针对鞭毛蛋白（fliC）基因的引物。>结果： >发现大量尿液样本（15毫升）最适合检测沙门氏菌血清型。尿液样本在40.9％的病例中被两种血清型混合感染，但血液（27.3％）和粪便（13.6％）较低。>结论：本研究得出结论：伤寒和慢性伤寒携带者的个体中混合感染的发生可能非常频繁，尽管最近报道的副伤寒A发病率上升可能并不真实。

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