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首页> 外文期刊>The Southeast Asian journal of tropical medicine and public health >APPLICATION OF QUANTITATIVE PCR FOR QUANTIZATION OF DENSOVIRUS GENOME
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APPLICATION OF QUANTITATIVE PCR FOR QUANTIZATION OF DENSOVIRUS GENOME

机译：定量PCR在齿状病毒基因组定量中的应用。

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摘要

Densovirus is classified as invertebrate virus belonging to the subfamily Densovirinae of Parvoviridae family. This group of viruses infects only insects and several densoviruses have been isolated from indigenous mosquitoes and mosquito cell lines. A number of mosquitoes, especially Aedes aegypti and Ae. albopictus are important vectors of viruses, which are the major causes of dengue hemorrhagic fever and yellow fever in humans. As densoviruses do not cause any pathology in humans, these viruses have been proposed to be a potential vector for use in biological control of mosquitoes and insects. We report the application of quantitative (q)PCR to determine the amount of densovirus genome in mosquito cell culture supernatant and mosquito. This method is simple, rapid and has a wide dynamic range, and therefore is likely to be useful and applicable in the determination of viral load of other viruses in a variety of biological specimens.
机译：登革病毒被归类为细小病毒科的登革病毒亚科的无脊椎动物病毒。这组病毒仅感染昆虫，并且已经从本地蚊子和蚊子细胞系中分离出了几种病毒。许多蚊子，尤其是埃及伊蚊和埃及伊蚊。白化病是病毒的重要载体，是人类登革热出血热和黄热病的主要原因。由于densoviruses不会在人类中引起任何病理，因此已提出这些病毒是用于蚊子和昆虫生物防治的潜在载体。我们报告了定量（q）PCR的应用，以确定在蚊细胞培养上清液和蚊中的densovirus基因组的数量。该方法简单，快速且动态范围广，因此可能在确定各种生物样本中其他病毒的病毒载量方面很有用和适用。

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