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首页> 外文期刊>The Veterinary Journal >Porcine adenovirus as a delivery system for swine vaccines and immunotherapeutics
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Porcine adenovirus as a delivery system for swine vaccines and immunotherapeutics

机译：猪腺病毒作为猪疫苗和免疫疗法的递送系统

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摘要

Porcine adenovirus (PAdV) has many qualities which make it an ideal choice for use as a delivery vector in swine. It is a low grade pathogen, present almost world-wide in a number of serotypes varying in their virulence and tissue tropism, which may allow for serotype specific vaccine targeting. PAdV is species specific having only been isolated from swine, reducing the possibility of its spread to other animals or man following administration. When engineered to contain a foreign gene, recombinant PAdV (rPAdV) can be grown to high titres in tissue culture cells making it cheap to produce. Knowledge of the complete nucleotide sequence of the PAdV genome has enabled rationally directed insertions of foreign genes which remain stably inserted in the genome and can be expressed at high levels following delivery to the target host. Importantly, recombinant PAdV can be administered by injection or by the oral route in feed or drinking water. We have delivered a range of antigens and immunomodulatory molecules to commercially available pigs using rPAdV and found it to be a very effective delivery system. Significantly, recombinant PAdV serotype 3 is highly effective as a delivery vehicle even when administered in the face of high levels of artificially induced serotype specific neutralising antibody to the vector.
机译：猪腺病毒（PAdV）具有许多特性，使其成为猪中传递载体的理想选择。它是一种低级病原体，其毒力和组织嗜性各不相同，并在全世界范围内以多种血清型出现，这可能使针对血清型的疫苗成为靶标。 PAdV是特定物种，仅从猪中分离出来，减少了给药后传播到其他动物或人的可能性。当工程改造为包含外源基因时，重组PAdV（rPAdV）可以在组织培养细胞中生长至高滴度，从而使其廉价生产。对PAdV基因组完整核苷酸序列的了解使得能够合理指导外源基因的插入，这些基因可以稳定地插入基因组中，并且可以在递送至目标宿主后以高水平表达。重要的是，重组PAdV可以通过注射或口服途径在饲料或饮用水中给药。我们已经使用rPAdV将一系列抗原和免疫调节分子传递给了商用猪，并发现这是一种非常有效的传递系统。值得注意的是，即使面对载体的高水平人工诱导的血清型特异性中和抗体，重组PAdV血清型3作为递送载体也是非常有效的。

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