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首页> 外文期刊>The Veterinary Journal >Constitutive apoptosis in equine peripheral blood neutrophils in vitro
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Constitutive apoptosis in equine peripheral blood neutrophils in vitro

机译：马外周血中性粒细胞体外本构性凋亡

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The aim of this study was to characterise constitutive apoptosis in equine peripheral blood neutrophils, including assessment of factors that potentially modulate neutrophil survival through alteration of the rate of constitutive apoptosis. Cells underwent spontaneous time-dependent constitutive apoptosis when aged in culture for up to 36 h, developing the structural and functional features of apoptosis observed in many cell types, including human neutrophils. Neutrophils undergoing apoptosis also had diminished zymosan activated serum (ZAS)-stimulated chemiluminescence, but maintained responsiveness to phorbol myristate acetate (PMA). The constitutive rate of equine neutrophil apoptosis was promoted by lipopolysaccharide (LPS), tumour necrosis factor a and phagocytosis of opsonised ovine erythrocytes, while it was inhibited by dexamethasone and ZAS (a source of C5a). Formyl-Met-Leu-Phe, leukotriene B-4, platelet activating factor and PMA had no demonstrable effect on equine neutrophil apoptosis. There was a difference between equine and human neutrophil apoptosis in response to LPS and the time-dependence of the response to dexamethasone. (C) 2014 The Authors. Published by Elsevier Ltd.
机译：这项研究的目的是表征马外周血中性粒细胞的组成性凋亡，包括评估可能通过改变组成性凋亡率来调节中性粒细胞存活的因素。当在培养物中陈化长达36小时时，细胞会经历自发的时间依赖性组成型凋亡，从而发展出在许多细胞类型（包括人类嗜中性粒细胞）中观察到的凋亡的结构和功能特征。经历凋亡的中性粒细胞也减少了酵母聚糖激活的血清（ZAS）刺激的化学发光，但保持了对佛波醇肉豆蔻酸酯乙酸（PMA）的响应性。脂多糖（LPS），肿瘤坏死因子a和调理绵羊红细胞的吞噬作用可提高马中性粒细胞凋亡的本构率，而地塞米松和ZAS（C5a的来源）则抑制了中性粒细胞凋亡。甲酰-Met-Leu-Phe，白三烯B-4，血小板活化因子和PMA对马中性粒细胞凋亡无明显作用。对LPS的反应马和中性粒细胞凋亡与地塞米松反应的时间依赖性之间存在差异。 （C）2014作者。由Elsevier Ltd.发布

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