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MEASUREMENT METHOD OF PERIPHERAL BLOOD NEUTROPHIL APOPTOSIS
MEASUREMENT METHOD OF PERIPHERAL BLOOD NEUTROPHIL APOPTOSIS
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机译：外周血中性神经细胞凋亡的测定方法
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摘要
PROBLEM TO BE SOLVED: To provide methods of measuring the ratio of peripheral blood neutrophil apoptosis, in which the ratio of in vivo neutrophil apoptosis is grasped rapidly and simply in real time, while minimizing the effect on the neutrophil after blood collecting.;SOLUTION: A method of measuring the ratio of peripheral blood neutrophil apoptosis comprises (a) a step in which neutrophils are separated from a biogenic blood sample and washed with phosphate buffered saline (PBS) to prepare a neutrophil suspension (phosphate buffered saline) with a concentration of 1×107/mL or more; (b) mixing the neutrophil suspension with the apoptosis Dye and subsequently culturing; (c) examining the cultured neutrophil suspension with a microscope using a hemocytometer and counting the ratio of dyed apoptosis positive neutrophils to the total count of the neutrophils in one visual field, wherein the centrifugation is performed for 3 to 10 minutes at 400 to 800 g, the concentration of the apoptosis Dye is 2 to 5%, and the culture is performed in 5% carbonic acid gas at 37°C for 8 to 10 minutes.;SELECTED DRAWING: Figure 3;COPYRIGHT: (C)2017,JPO&INPIT
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