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首页> 外文期刊>The Philippine Journal of Crop Science >ENDOPHYTIC BACTERIA ASSOCIATED WITH SWEET POTATO & THEIR INTERACTION UNDER CO-CULTURE CONDITIONS
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ENDOPHYTIC BACTERIA ASSOCIATED WITH SWEET POTATO & THEIR INTERACTION UNDER CO-CULTURE CONDITIONS

机译：共培养条件下甜薯与内生细菌的相互作用

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Endophytic bacteria have been reported to help increase crop yield by producing plant growth-promoting substances and feeing atmospheric nitrogen (N_2). Previous studies have shown a possible interaction between sweet potato and N_2-fbeing (diazotrophic) endophytes. In this study, we isolated, characterized, and identified the endophytic bacteria associated with sweet potato. Moreover, the interaction between N_2-fixing and non-diazotrophic isolates was studied under co-culture conditions. The occurrence of diazotrophic endophytes in stem portions of sweet potato was examined by stem piece-incubation and acetylene reduction activity (SPI-ARA) method using a semi-solid (1.8 grams agar/L) modified Rennie (MR) medium. Surface-sterilized and thinly sliced (1-2 mm) sweet potato stem samples were incubated in test tubes with semi-solid MR medium. The tubes were assayed for acetylene reduction activity (ARA) 5 days after incubation at 30 deg C. Twelve isolates were obtained from solid MR agar plates (20 gagar/L) that were inoculated with a loop of semi-solid MR medium from AR4 positive tubes. The ARA test of the 12 isolates showed that 9 strains were diazotrophic and 3 were non-diazotrophic. Using the API 20E diagnostic kit, 4 diazotrophic isolates wereidentified ay strains of Pantoea spp. and 5 isolates as Klebsiellu spp. The non-diazotrophs were strains of Enterobacter spp. The diazotrophic strain MY1 and non-diazotrophic strain MY2 were selected and identified to the species level by full sequenceanalysis of the 16S rRNA gene. The results showed that MY I had 99.2% similarity to Pantoea agglomerans A TCC 2 7155 and MY2 had 99.5% similarity to Enterobacter asburiae ATCC 35953. We also observed beneficial interaction between diazotrophic endophyteP. agglomerans MY I and nondiazotrophic endophyte E. asburiae MY2. The co-culture of MY 1 with MY2 in semi-solid MR medium resulted in an increased ARA of MY1. Co-culture effect, however, was markedly reduced when either sucrose or Malate was removed from the medium, indicating that the increase in ARA is probably due to the strains' efficient use of both sucrose and Malate in the AIR medium.
机译：据报道，内生细菌可通过产生促进植物生长的物质和补充大气中的氮（N_2）来帮助提高作物产量。先前的研究表明，甘薯和N_2-fbeing（营养缺陷型）内生菌之间可能存在相互作用。在这项研究中，我们分离，表征和鉴定了与甘薯有关的内生细菌。此外，在共培养条件下研究了固氮菌与非营养菌分离株之间的相互作用。使用半固体（1.8克琼脂/升）改良的Rennie（MR）培养基，通过茎片温育和乙炔还原活性（SPI-ARA）方法检查了甘薯茎部中重氮营养内生菌的存在。经过表面灭菌并切成薄片（1-2毫米）的甘薯茎样品在装有半固体MR培养基的试管中孵育。在30摄氏度下孵育5天后，对试管的乙炔还原活性（ARA）进行了分析。从固体MR琼脂平板（20 gagar / L）中获得了十二个分离株，将其接种了来自AR4阳性的半固体MR培养基环管。对12株分离株的ARA试验表明，9株是重氮营养的，3株是非重营养的。使用API​​ 20E诊断试剂盒，通过Pantoea spp菌株鉴定了4个重氮营养分离株。和5个分离株，如Klebsiellu spp。非固氮菌是肠杆菌属的菌株。通过对16S rRNA基因进行全序列分析，选择重氮营养菌株MY1和非重营养菌株MY2并鉴定到种水平。结果表明，MY I与泛集聚体A TCC 2 7155的相似度为99.2％，MY2与白色肠杆菌ATCC 35953的相似度为99.5％。我们还观察到重氮营养内生菌之间的有益相互作用。团聚体MY I和非营养型内生真菌白霉菌MY2。在半固态MR培养基中将MY 1与MY2共培养会导致MY1的ARA升高。然而，当从培养基中除去蔗糖或苹果酸时，共培养效果显着降低，表明ARA的增加可能是由于菌株在AIR培养基中有效利用了蔗糖和苹果酸。

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