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首页> 外文期刊>Thorax: The Journal of the British Thoracic Society >Neutrophil TLR4 expression is reduced in the airways of infants with severe bronchiolitis.
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Neutrophil TLR4 expression is reduced in the airways of infants with severe bronchiolitis.

机译：患有严重细支气管炎的婴儿的气道中性粒细胞TLR4表达降低。

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BACKGROUND: In respiratory syncytial virus (RSV) bronchiolitis, neutrophils account for >80% of cells recovered from the airways in bronchoalveolar lavage (BAL) fluid. This study investigated neutrophil activation and Toll-like receptor (TLR) expression in the blood and lungs of infants with severe RSV bronchiolitis. METHODS: BAL fluid and (blood) samples were collected from 24 (16) preterm and 23 (15) term infants ventilated with RSV bronchiolitis, and 12 (8) control infants. Protein levels and mRNA expression of CD11b, myeloperoxidase (MPO) and TLRs 2, 4, 7, 8 and 9 were measured in neutrophils. RESULTS: Blood neutrophils had more CD11b in preterm and term infants with RSV bronchiolitis than control infants (p<0.025) but similar amounts of MPO. BAL fluid neutrophils from infants with RSV bronchiolitis had greater amounts of CD11b and MPO than blood neutrophils and BAL fluid neutrophils from controls (p<0.01). Blood neutrophils from term infants with RSV bronchiolitis had less total TLR4 protein than preterm infants with RSV bronchiolitis (p = 0.005), and both had less than controls (p<0.04). Total TLR4 for each group was greater in BAL fluid neutrophils than in blood neutrophils. Blood neutrophils from preterm infants with RSV bronchiolitis had greater TLR4 mRNA expression than term infants with RSV bronchiolitis (p = 0.005) who had similar expression to controls (p = 0.625). CONCLUSIONS: In infants with severe RSV bronchiolitis, neutrophil activation starts in the blood and progresses as they are recruited into the airways. Total neutrophil TLR4 remains low in both compartments. TLR4 mRNA expression is unimpaired. This suggests that neutrophil TLR4 expression is deficient in these infants, which may explain why they develop severe RSV bronchiolitis.
机译：背景：在呼吸道合胞病毒（RSV）细支气管炎中，中性粒细胞占支气管肺泡灌洗液（BAL）中从气道中回收的细胞的80％以上。这项研究调查了严重RSV细支气管炎婴儿的血液和肺中嗜中性粒细胞活化和Toll样受体（TLR）的表达。方法：从24例RSV细支气管炎通气的早产婴儿和23例（15）足月婴儿和12例（8）对照婴儿中收集BAL液和（血液）样本。测量中性粒细胞中CD11b，髓过氧化物酶（MPO）和TLR 2、4、7、8和9的蛋白水平和mRNA表达。结果：RSV细支气管炎早产儿和足月儿的血液中性粒细胞CD11b高于对照组（p <0.025），但MPO含量相似。 RSV毛细支气管炎婴儿的BAL液体中性粒细胞比对照组的血液中性粒细胞和BAL液体中性粒细胞具有更多的CD11b和MPO（p <0.01）。 RSV细支气管炎足月儿的血液中性粒细胞总TLR4蛋白比RSV细支气管炎早产儿（p = 0.005），且均低于对照组（p <0.04）。每组中BAL液体中性粒细胞的总TLR4大于血液中性粒细胞。 RSV细支气管炎早产儿的血液嗜中性粒细胞比正常对照组的RSV细支气管炎的早产儿（p = 0.005）具有更高的TLR4 mRNA表达（p = 0.625）。结论：患有严重RSV细支气管炎的婴儿，嗜中性粒细胞激活开始于血液，并随着其进入呼吸道而发展。在两个隔室中总中性粒细胞TLR4保持较低水平。 TLR4 mRNA表达未受损。这表明在这些婴儿中嗜中性粒细胞TLR4表达不足，这可能解释了为什么他们发展为严重的RSV细支气管炎。

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