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Anomalous migration of DNA

机译:DNA的异常迁移

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摘要

A quantitative model for the electrophoretic mobility of very small fragments of A-DNA and Z-DNA in gel is described, The model is a synthesis of counterion condensation features of ionic oligomers, screening of the hydrodynamic interactions of an oligomer, and a suitable extension of the Ogstron pore-size distribution model of gel migration. Anomalous migration of very small fragments of A- and Z-DNA is predicted. The electrophoretic mobility is an increasing function of its length for small fragments, reaches a maximum and then starts to slow down for larger fragments. For very small sequence lengths studied here, A-DNA migrates faster than Z-DNA of the same length in polyacrylamide gel. (C) 2000 Elsevier Science B.V. All rights reserved. [References: 22]
机译:描述了凝胶中A-DNA和Z-DNA极小片段电泳迁移的定量模型,该模型是离子低聚物的抗衡离子缩合特征的合成,低聚物的水动力相互作用的筛选以及合适的扩展迁移的Ogstron孔径分布模型的建立。可以预料到非常小的A-和Z-DNA片段的异常迁移。对于小片段,电泳迁移率是其长度的增加函数,最大时达到最大值,然后开始减慢。对于此处研究的非常小的序列长度,在聚丙烯酰胺凝胶中,A-DNA的迁移速度要快于相同长度的Z-DNA。 (C)2000 Elsevier Science B.V.保留所有权利。 [参考:22]

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