...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Theriogenology >Comparative quantitative assessment of GnRH- and LH-receptor mRNA expression in the urinary tract of sexually intact and spayed female dogs
【24h】

Comparative quantitative assessment of GnRH- and LH-receptor mRNA expression in the urinary tract of sexually intact and spayed female dogs

机译：比较完整的雌性和雌性狗的尿路中GnRH和LH受体mRNA表达的定量比较

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Ovariectomy interrupts the regulatory loop in the hypothalamus-pituitary-gonad axis, leading to a several-fold increase in gonadotropin levels. This rise in hormonal secretion may play a causal role in ovariectomy-related urinary incontinence. The purpose of this study was to examine the effect of ovariectomy in bitches on the expression of GnRH- and LH-receptors in the lower urinary tract, and assess the relationship between receptor expression and plasma gonadotropin concentrations. Plasma gonadotropins were measured in 37 client-owned bitches. Biopsies were harvested from the mid-ventral bladder wall in all dogs, and from nine further locations within the lower urinary tract in 17 of the 37 animals. Messenger RNA of the LH and GnRH receptors was quantified using RT-PCR with the TaqMan Universal PCR Master Mix. Gonadotropins were measured with a canine-specific FSH-immunoradiometric assay and LH-radioimmunoassay. The hierarchical mixed ANOVA model using MINITAB, Mann-Whitney U-test, unpaired means comparison and linear regressions using StatView were applied for statistical analyses. Messenger RNA for both receptors was detected in all biopsy samples. Age was negatively correlated to mRNA expression of the LH and the GnRH receptors. A relationship between the mRNA values and the plasma gonadotropin concentrations was not established. Evaluation of results within each of the biopsy locations revealed greater LH-receptor expression in the proximal second quarter of the urethra in spayed bitches than in intact bitches (P=0.0481). Increased mRNA expression of LH receptors in this location could possibly play a role in the decrease in closing pressure of the urethra following ovariectomy.
机译：卵巢切除术中断了下丘脑-垂体-性腺轴的调节环，导致促性腺激素水平增加了几倍。激素分泌的增加可能在卵巢切除相关的尿失禁中起因果作用。这项研究的目的是检查雌性卵巢切除对下尿路GnRH和LH受体表达的影响，并评估受体表达与血浆促性腺激素浓度之间的关系。在37个客户拥有的母犬中测量血浆促性腺激素。从所有犬的腹中部膀胱壁以及37只动物中的17只从下泌尿道内的另外九个位置采集活组织检查。使用TaqMan Universal PCR Master Mix使用RT-PCR对LH和GnRH受体的信使RNA进行定量。促性腺激素通过犬特异性FSH免疫放射测定法和LH放射免疫测定法进行测量。使用MINITAB的分层混合ANOVA模型，Mann-Whitney U检验，未配对均值比较和使用StatView的线性回归进行了统计分析。在所有活检样本中均检测到两种受体的信使RNA。年龄与LH和GnRH受体的mRNA表达呈负相关。 mRNA值与血浆促性腺激素浓度之间的关系尚未建立。对每个活检部位的结果进行评估后发现，与完整母犬相比，小母犬在尿道近端第二季度的LH受体表达更高（P = 0.0481）。 LH受体在该位置的mRNA表达增加可能在卵巢切除术后尿道闭合压力降低中起作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号