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首页> 外文期刊>Thrombosis Research: An International Journal on Vascular Obstruction, Hemorrhage and Hemostasis >Alteration of the fibrinogen molecule and its phosphorylation state in myocardial infarction patients undergoing thrombolytic treatment.
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Alteration of the fibrinogen molecule and its phosphorylation state in myocardial infarction patients undergoing thrombolytic treatment.

机译：接受溶栓治疗的心肌梗死患者中纤维蛋白原分子的变化及其磷酸化状态。

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Fibrinogen was purified by protamine-agarose chromatography from plasma from three patients after their submission to hospital due to acute myocardial infarction. The total amount of phosphate bound to fibrinogen and the concentration of fibrinogen was determined in samples withdrawn immediately after submission and after thrombolytic treatment. Streptokinase treatment almost totally removed circulating fibrinogen while recombinant tissue plasminogen activator spared much of it. In patients treated with streptokinase, the new circulating fibrinogen was homogeneous according to the single alpha-band seen after sodium dodecyl sulphate polyacrylamide gel electrophoresis analysis under reducing conditions, whereas fibrinogen from the recombinant tissue plasminogen activator-treated patient as well as healthy controls exhibited two alpha-bands in the 66-kDa region. The molar ratios of phosphate to fibrinogen of healthy controls and commercial fibrinogen were 0.82 (+/-0.04) and 0. 87 (+/-0.05), respectively. For two streptokinase-treated patients the degree of phosphorylation increased threefold from a normal range of 0.97 (+/-0.11) and 0.67 (+/-0.09) mol/mol fibrinogen before treatment to 3.33 (+/-0.32) and 1.86 (+/-0.17) mol/mol in newly formed fibrinogen on day 1. Recombinant tissue plasminogen activator treatment led to a smaller increase in phosphorylation, from 1.14 (+/-0.13) pretreatment to 1.65 (+/-0.11) after treatment on day 1. In conclusion we show in this report that after streptokinase treatment of patients with acute myocardial infarction, the new Aalpha-chain of fibrinogen was a homogeneous single 66-kDa band on sodium dodecyl sulphate polyacrylamide gel electrophoresis under reducing conditions and that the degree of phosphorylation of plasma fibrinogen was elevated, approaching the theoretical limit of 4 mol phosphate/mol fibrinogen.
机译：在因急性心肌梗塞入院后，从三名患者的血浆中通过鱼精蛋白-琼脂糖层析纯化了纤维蛋白原。在提交后和溶栓治疗后立即取出的样品中测定与纤维蛋白原结合的磷酸盐总量和纤维蛋白原浓度。链激酶治疗几乎完全清除了循环中的纤维蛋白原，而重组组织纤溶酶原激活剂则节省了大部分。在链激酶处理的患者中，在还原条件下十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析后，根据单个α带，新的循环纤维蛋白原是均匀的，而重组组织纤溶酶原激活物治疗的患者以及健康对照组的纤维蛋白原表现出两个66 kDa区域的alpha波段。健康对照和商业纤维蛋白原的磷酸盐与纤维蛋白原的摩尔比分别为0.82（+/- 0.04）和0. 87（+/- 0.05）。对于两名接受链激酶治疗的患者，磷酸化程度从治疗前的正常范围0.97（+/- 0.11）和0.67（+/- 0.09）mol / mol纤维蛋白原增加了三倍，至治疗前的3.33（+/- 0.32）和1.86（+ /-0.17）mol / mol在第1天刚形成的纤维蛋白原中。重组组织纤溶酶原激活剂处理导致磷酸化的增加较小，从预处理的1.14（+/- 0.13）到治疗后的第1天的1.65（+/- 0.11）总之，我们在本报告中表明，在链激酶治疗急性心肌梗死患者后，纤维蛋白原的新Aalpha链在十二烷基硫酸钠聚丙烯酰胺凝胶电泳上在还原条件下呈均一的66 kDa均匀条带，并且磷酸化程度血浆纤维蛋白原的含量升高，接近4 mol磷酸盐/ mol纤维蛋白原的理论极限。

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