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首页> 外文期刊>Tissue engineering, Part A >The effect of simulated microgravity on human mesenchymal stem cells cultured in an osteogenic differentiation system: a bioinformatics study.
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The effect of simulated microgravity on human mesenchymal stem cells cultured in an osteogenic differentiation system: a bioinformatics study.

机译：模拟微重力对成骨分化系统中培养的人间充质干细胞的影响：一项生物信息学研究。

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One proposed strategy for bone regeneration involves ex vivo tissue engineering, accomplished using bone-forming cells, biodegradable scaffolds, and dynamic culture systems, with the goal of three-dimensional tissue formation. Rotating wall vessel bioreactors generate simulated microgravity conditions ex vivo, which lead to cell aggregation. Human mesenchymal stem cells (hMSCs) have been extensively investigated and shown to possess the potential to differentiate into several cell lineages. The goal of the present study was to evaluate the effect of simulated microgravity on all genes expressed in hMSCs, with the underlying hypothesis that many important pathways are affected during culture within a rotating wall vessel system. Gene expression was analyzed using a whole genome microarray and clustering with the aid of the National Institutes of Health's Database for Annotation, Visualization and Integrated Discovery database and gene ontology analysis. Our analysis showed 882 genes that were downregulated and 505 genes that were upregulated after exposure to simulated microgravity. Gene ontology clustering revealed a wide variety of affected genes with respect to cell compartment, biological process, and signaling pathway clusters. The data sets showed significant decreases in osteogenic and chondrogenic gene expression and an increase in adipogenic gene expression, indicating that ex vivo adipose tissue engineering may benefit from simulated microgravity. This finding was supported by an adipogenic differentiation assay. These data are essential for further understanding of ex vivo tissue engineering using hMSCs.
机译：一种提出的骨再生策略涉及离体组织工程，该工程使用形成骨的细胞，可生物降解的支架和动态培养系统来实现，以实现三维组织形成为目标。旋转壁容器生物反应器在体外产生模拟的微重力条件，从而导致细胞聚集。人间充质干细胞（hMSCs）已被广泛研究，并显示具有分化为几种细胞谱系的潜力。本研究的目的是评估模拟微重力对hMSCs中表达的所有基因的影响，其基本假设是在旋转壁容器系统内培养期间许多重要途径均受到影响。使用全基因组微阵列分析基因表达，并借助美国国立卫生研究院的注释，可视化和集成发现数据库和基因本体分析进行聚类。我们的分析显示，在受到模拟微重力作用后，有882个基因被下调，而有505个基因被上调。基因本体聚类揭示了有关细胞区室，生物学过程和信号通路簇的多种受影响的基因。数据集显示成骨和软骨形成基因表达显着减少，而成脂基因表达则增加，表明离体脂肪组织工程可能会受益于模拟微重力。这一发现得到了成脂分化测定的支持。这些数据对于进一步了解使用hMSC的离体组织工程至关重要。

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