• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Tissue engineering, Part C. Methods >The quest for an optimized protocol for whole-heart decellularization: a comparison of three popular and a novel decellularization technique and their diverse effects on crucial extracellular matrix qualities.
【24h】

The quest for an optimized protocol for whole-heart decellularization: a comparison of three popular and a novel decellularization technique and their diverse effects on crucial extracellular matrix qualities.

机译：寻求针对全心脏脱细胞的优化方案：三种流行的和新型脱细胞技术的比较及其对关键细胞外基质质量的不同影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Decellularized cardiac extracellular matrix (ECM) has been introduced as a template for cardiac tissue engineering, providing the advantages of a prevascularized scaffold that mimics native micro- and macroarchitecture to a degree difficult to achieve with synthetic materials. Nonetheless, the decellularization protocols used to create acellular myocardial scaffolds vary widely throughout the literature. In this study we performed a direct comparison of three previously described protocols while introducing and evaluating a novel, specifically developed fourth protocol, by decellularizing whole rat hearts through software-controlled automatic coronary perfusion. Although all protocols preserved the macroarchitecture of the hearts and all resulting scaffolds could successfully be reseeded with C2C12 myoblasts, assessing their biocompatibility for three-dimensional in vitro studies, we found striking differences concerning the microcomposition of the ECM scaffolds on a histological and biochemical level. While laminin could still be detected in all groups, other crucial ECM components, like elastin and collagen IV, were completely removed by at least one of the protocols. Further, only three protocols maintained a glycosaminoglycan content comparable to native tissue, whereas the remaining DNA content within the ECM varied highly throughout all four tested protocols. This study showed that the degree of acellularity and resulting ECM composition of decellularized myocardial scaffolds strongly differs depending on the decellularization protocol.
机译：脱细胞的心脏细胞外基质（ECM）已作为心脏组织工程的模板引入，提供了预血管化支架的优势，该支架模仿了天然的微结构和宏观结构，难以用合成材料实现。然而，在整个文献中，用于产生脱细胞心肌支架的脱细胞方案均存在很大差异。在这项研究中，我们通过软件控制的自动冠状动脉灌注使整个大鼠心脏脱细胞，同时引入和评估了一种新的，专门开发的第四种方案，同时对三种上述方案进行了直接比较。尽管所有协议均保留了心脏的宏结构，并且所有产生的支架均可成功植入C2C12成肌细胞，评估其在3D体外研究中的生物相容性，但我们在组织学和生化水平上发现了关于ECM支架微组成的显着差异。尽管仍然可以在所有组中检测到层粘连蛋白，但至少其中一种方案已完全清除了其他重要的ECM成分，例如弹性蛋白和IV型胶原。此外，只有三个方案保持了与天然组织相当的糖胺聚糖含量，而在所有四个测试方案中，ECM中的其余DNA含量变化很大。这项研究表明，脱细胞的心肌支架的脱细胞度和所得ECM组成随脱细胞方案的不同而有很大差异。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号