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首页> 外文期刊>Tissue engineering, Part C. Methods >Chitosan scaffolds containing hyaluronic acid for cartilage tissue engineering.
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Chitosan scaffolds containing hyaluronic acid for cartilage tissue engineering.

机译：含有透明质酸的壳聚糖支架，用于软骨组织工程。

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Scaffolds derived from natural polysaccharides are very promising in tissue engineering applications and regenerative medicine, as they resemble glycosaminoglycans in the extracellular matrix (ECM). In this study, we have prepared freeze-dried composite scaffolds of chitosan (CHT) and hyaluronic acid (HA) in different weight ratios containing either no HA (control) or 1%, 5%, or 10% of HA. We hypothesized that HA could enhance structural and biological properties of CHT scaffolds. To test this hypothesis, physicochemical and biological properties of CHT/HA scaffolds were evaluated. Scanning electron microscopy micrographs, mechanical properties, swelling tests, enzymatic degradation, and Fourier transform infrared (FTIR) chemical maps were performed. To test the ability of the CHT/HA scaffolds to support chondrocyte adhesion and proliferation, live-dead and MTT assays were performed. Results showed that CHT/HA composite scaffolds are noncytotoxic and promote cell adhesion. ECM formation was further evaluated with safranin-O and alcian blue staining methods, and glycosaminoglycan and DNA quantifications were performed. The incorporation of HA enhanced cartilage ECM production. CHT/5HA had a better pore network configuration and exhibited enhanced ECM cartilage formation. On the basis of our results, we believe that CHT/HA composite matrixes have potential use in cartilage repair.
机译：源自天然多糖的支架在组织工程应用和再生医学中非常有前途，因为它们类似于细胞外基质（ECM）中的糖胺聚糖。在这项研究中，我们制备了不同重量比的壳聚糖（CHT）和透明质酸（HA）的冻干复合支架，其中不含HA（对照）或1％，5％或10％的HA。我们假设HA可以增强CHT支架的结构和生物学特性。为了验证该假设，评估了CHT / HA支架的理化和生物学特性。进行了扫描电子显微镜显微照片，机械性能，溶胀测试，酶促降解和傅立叶变换红外（FTIR）化学图。为了测试CHT / HA支架支持软骨细胞粘附和增殖的能力，进行了活死和MTT测定。结果表明，CHT / HA复合支架无细胞毒性，可促进细胞粘附。用番红O和阿尔辛蓝染色方法进一步评估了ECM的形成，并对糖胺聚糖和DNA进行了定量。 HA的掺入增强了软骨ECM的产生。 CHT / 5HA具有更好的孔网络配置，并显示出增强的ECM软骨形成。根据我们的结果，我们相信CHT / HA复合基质在软骨修复中具有潜在用途。

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