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首页> 外文期刊>Tissue engineering, Part C. Methods >Effects of low-amplitude, high-frequency vibrations on proliferation and differentiation of SAOS-2 human osteogenic cell line.
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Effects of low-amplitude, high-frequency vibrations on proliferation and differentiation of SAOS-2 human osteogenic cell line.

机译：低振幅，高频振动对SAOS-2人成骨细胞系增殖和分化的影响。

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摘要

The aim of the work was to understand the consequences of low-amplitude, high-frequency vibrations on proliferation and differentiation of SAOS-2 cells (sarcoma osteogenetic), an osteoblastic and tumorigenic cell line. We realized a bioreactor composed of an eccentric motor that produces a displacement of 11 mm at frequencies between 1 and 120 Hz on a plate connected to the motor. The cultures of SAOS-2 cells were fixed on the plate, and the linear acceleration provoked by the motor to the cultures was measured. We used 30 Hz as stimulating frequency after a preliminary test on the effect of different frequencies on differentiation of cells. Afterward, SAOS-2 cells were stimulated with 30 Hz for different durations, every day for 4 days. The expression of some genes involved in the differentiation process was analyzed first with a reverse transcriptase-polymerase chain reaction and afterward with a real-time polymerase chain reaction on the most expressed genes. Moreover, the proliferation of cells was evaluated. The results suggest a strong increase in the expression of the genes involved in tissue differentiation in the treated groups with respect to the controls. On the other hand, the proliferation seems to be slowed down, so probably the acceleration perceived by the mechanosensors of the cells changes the cellular cycle by blocking the duplication to early differentiate toward bone tissue.
机译：这项工作的目的是了解低振幅，高频振动对成骨细胞和致瘤细胞系SAOS-2细胞（肉瘤成骨）增殖和分化的影响。我们实现了一个由偏心电机组成的生物反应器，该偏心电机在与电机相连的板上以1至120 Hz的频率产生11 mm的位移。将SAOS-2细胞的培养物固定在平板上，并测量由马达引起的线性加速度。在对不同频率对细胞分化的影响进行初步测试后，我们使用30 Hz作为刺激频率。之后，每天以30 Hz的频率刺激SAOS-2细胞持续4天。首先通过逆转录酶-聚合酶链反应，然后对大多数表达的基因进行实时聚合酶链反应，分析与分化过程有关的一些基因的表达。此外，评估了细胞的增殖。结果表明，与对照组相比，治疗组中参与组织分化的基因表达明显增加。另一方面，增殖似乎被减慢了，因此可能由细胞的机械传感器感知到的加速度通过阻止复制而向骨组织早期分化，从而改变了细胞周期。

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