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A MICROCALORIMETRIC METHOD TO STUDY THE ACTIVATION OF MURINE PERITONEAL MACROPHAGES

机译:微量热法研究小鼠腹膜巨噬细胞的活化

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摘要

A novel microcalorimetric method was used to continuously follow changes in the overall metabolic energy expenditure of rat peritoneal macrophages during PMA activation. Macrophages were isolated from: (1) healthy animals; (2) animals suffering from D-galactosamine induced acute liver injury (ALI); and (3) animals which sustained a glycerol induced renal failure (RF). Results from measurements on macrophages taken from healthy animals showed that microcalorimetric findings were reproducible and consistent with luminometric recordings of released reactive oxygen species. However, discrepancies were observed between results obtained with the two techniques in the ALI and RF groups, indicating differences in the mechanism by which these two conditions affect the macrophage system. [References: 11]
机译:一种新颖的微量量热法用于在PMA激活过程中连续跟踪大鼠腹膜巨噬细胞的总代谢能消耗。巨噬细胞分离自:(1)健康动物; (2)患有D-半乳糖胺的动物引起急性肝损伤(ALI); (3)持续存在甘油诱导的肾衰竭(RF)的动物。对健康动物的巨噬细胞进行测量的结果表明,微量量热法的发现是可重复的,并且与释放的活性氧的发光记录一致。但是,在ALI和RF组中,用两种技术获得的结果之间观察到差异,表明这两种条件影响巨噬细胞系统的机制存在差异。 [参考:11]

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