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首页> 外文期刊>Transactions of the Royal Society of Tropical Medicine and Hygiene >Evaluation of newer diagnostic methods for the detection and differentiation of Entamoeba histolytica in an endemic area.
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Evaluation of newer diagnostic methods for the detection and differentiation of Entamoeba histolytica in an endemic area.

机译：评估在地方病地区检测和区分溶组织性变形杆菌的新型诊断方法。

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摘要

We evaluated the colorimetric polymerase chain reaction (PCR)-based method for the detection and differentiation of Entamoeba spp. and compared the efficacy of E. histolytica-specific antigen detection in faeces with the detection of specific antibodies to E. histolytica-specific antigen in faeces, by enzyme-linked immunosorbent assay. Faecal samples were obtained from patients attending hospital in Chandigarh, India, from March 2001 to February 2002. The PCR-based colorimetric method was found to be the most sensitive (100%) and it could differentiate between pathogenic and non-pathogenic Entamoeba spp. The present study also emphasized that the antigen detection system may prove to be a better diagnostic tool than the antibody detection system in endemic areas.
机译：我们评估了基于比色聚合酶链反应（PCR）的Entamoeba spp的检测和区分方法。并通过酶联免疫吸附法比较了粪便中组织溶酶原特异性抗原检测与粪便中针对组织溶酶原特异性抗原的特异性抗体检测的效果。从2001年3月至2002年2月从印度昌迪加尔的医院就诊的患者中采集粪便样品。发现基于PCR的比色法是最灵敏的（100％），可以区分致病性和非致病性的Entamoeba spp。本研究还强调，在流行地区，与抗体检测系统相比，抗原检测系统可能被证明是更好的诊断工具。

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