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首页> 外文期刊>Toxicology: An International Journal Concerned with the Effects of Chemicals on Living Systems >Vanin-1; a potential biomarker for nephrotoxicant-induced renal injury.
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Vanin-1; a potential biomarker for nephrotoxicant-induced renal injury.

机译：Vanin-1;潜在的肾毒性生物标志物。

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Because traditional markers for detecting renal injury are generally insensitive and nonspecific, we tried to identify some useful biomarkers. Microarray analyses and quantitative real-time PCR using human renal tubular cells showed that the mRNA expression of VNN-1 which encodes vanin-1, increased after the exposure of these cells to organic solvents (allyl alcohol, ethylene glycol, formaldehyde, chloroform, and phenol) for 24h. The mRNA levels of other inflammation-related molecules such as monocyte chemoattractant protein 1 (MCP-1) and kidney injury molecule-1 (KIM-1) also increased after the exposure to organic solvents, although their elevations were slower than that of vanin-1. In rats treated with ethylene glycol for 3 weeks, tubular injury was detected by histological examination, but not by traditional biomarkers including serum creatinine and urinary N-acetyl-beta-glucosaminidase. The mRNA levels of vanin-1 and Kim-1, but not MCP-1, significantly elevated in the renal cortices of ethylene glycol-exposed rats. On immunofluorescence analyses, vanin-1 signal was detected specifically in the renal tubules with a remarkable expression in the ethylene glycol-treated rats. As a result, compared with control group, higher urinary and serum concentrations of vanin-1 were observed in the ethylene glycol-treated group. These results suggest that vanin-1 is a useful and rapid biomarker for renal tubular injury induced by organic solvents.
机译：由于用于检测肾损伤的传统标记通常不敏感且不具特异性，因此我们尝试鉴定一些有用的生物标记。使用人肾小管细胞进行的微阵列分析和定量实时PCR显示，编码Vanin-1的VNN-1的mRNA表达在这些细胞暴露于有机溶剂（烯丙醇，乙二醇，甲醛，氯仿和苯酚）24小时。暴露于有机溶剂后，其他与炎症相关的分子（例如单核细胞趋化蛋白1（MCP-1）和肾损伤分子1（KIM-1））的mRNA水平也有所升高，尽管它们的升高速度低于Vanin- 1。在接受乙二醇治疗3周的大鼠中，通过组织学检查发现了肾小管损伤，但传统的生物标志物（包括血清肌酐和尿液中的N-乙酰基-β-氨基葡萄糖苷酶）未检测到肾小管损伤。在暴露于乙二醇的大鼠的肾皮质中，vanin-1和Kim-1的mRNA水平显着升高，而MCP-1则没有。在免疫荧光分析中，在肾小管中特异性检测到Vanin-1信号，在经乙二醇处理的大鼠中表达显着。结果，与对照组相比，在乙二醇治疗组中观察到较高的尿液和血清vanin-1浓度。这些结果表明，vanin-1是有机溶剂诱导的肾小管损伤的有用且快速的生物标志物。

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