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首页> 外文期刊>Toxicology: An International Journal Concerned with the Effects of Chemicals on Living Systems >Validation of an LDH assay for assessing nanoparticle toxicity.
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Validation of an LDH assay for assessing nanoparticle toxicity.

机译：用于评估纳米粒子毒性的LDH分析的验证。

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Studies showed that certain cytotoxicity assays were not suitable for assessing nanoparticle (NP) toxicity. We evaluated a lactate dehydrogenase (LDH) assay for assessing copper (Cu-40, 40nm), silver (Ag-35, 35nm; Ag-40, 40nm), and titanium dioxide (TiO(2)-25, 25nm) NPs by examining their potential to inactivate LDH and interference with beta-nicotinamide adenine dinucleotide (NADH), a substrate for the assay. We also performed a dissolution assay for some of the NPs. We found that the copper NPs, because of their high dissolution rate, could interfere with the LDH assay by inactivating LDH. Ag-35 could also inactivate LDH probably because of the carbon matrix used to cage the particles during synthesis. TiO(2)-25 NPs were found to adsorb LDH molecules. In conclusion, NP interference with the LDH assay depends on the type of NPs and the suitability of the assay for assessing NP toxicity should be examined case by case.
机译：研究表明某些细胞毒性试验不适合评估纳米颗粒（NP）毒性。我们评估了乳酸脱氢酶（LDH）测定法，以通过以下方法评估铜（Cu-40，40nm），银（Ag-35，35nm; Ag-40，40nm）和二氧化钛（TiO（2）-25，25nm）NP。检查其使LDH失活的潜力以及对β-烟酰胺腺嘌呤二核苷酸（NADH）（该检测底物）的干扰。我们还对一些NP进行了溶出度测定。我们发现，由于铜NP的高溶解速度，它们可以通过使LDH失活而干扰LDH分析。 Ag-35也可能使LDH失活，这可能是由于在合成过程中用于笼罩颗粒的碳基质。 TiO（2）-25 NP被发现吸附LDH分子。总之，NP对LDH分析的干扰取决于NP的类型，应逐案检查该分析评估NP毒性的适用性。

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