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首页> 外文期刊>Transactions of the American Ophthalmological Society. >Anterior segment alterations and comparative aqueous humor proteomics in the buphthalmic rabbit (an American Ophthalmological Society thesis).
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Anterior segment alterations and comparative aqueous humor proteomics in the buphthalmic rabbit (an American Ophthalmological Society thesis).

机译：眼科兔子的眼前节改变和房水比较蛋白质组学（美国眼科学会论文）。

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To use an integrated proteohistologic approach to gain insight into the anterior segment alterations in the buphthalmic rabbit.Eyes from 2- and 5-year-old buphthalmic and normal rabbits (n=20) were studied histologically. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) of aqueous humor (AH) was used to determine differential protein expression between animal groups. Western blot and immunohistochemistry were performed on selected differentially expressed proteins identified by LC-MS/MS.The buphthalmic rabbits manifested a mild clinical phenotype with typical angle anomalies that appeared progressive by histology. Significantly thickened Descemet's membrane (DM) and anterior lens capsule in all buphthalmic rabbits showed increased fibronectin and collagen-IV immunolabeling. LC-MS/MS applying stringent filtering criteria revealed significant differential expression of several AH proteins in these rabbits. The protein of interest in the 2-year-old group was histidine-rich glycoprotein, and those in the 5-year-old group included alpha-2-HS-glycoprotein, clusterin, apolipoprotein E, interphotoreceptor retinoid-binding protein, transthyretin, cochlin, gelsolin, haptoglobin, hemopexin, and beta-2 microglobulin. The proteomic data for selected proteins was validated by Western blot and immunohistochemistry. A wide range of functional groups were affected by the altered AH proteins. These included extracellular matrix modulation, regulation of apoptosis, oxidative stress, and protein transport.Multiple anterior segment alterations were histologically identified in the buphthalmic rabbits that showed progressive changes with age. The differentially expressed AH proteins in these rabbits suggest a multifunctional role for AH in modulating pathologic changes in DM, anterior lens capsule, and the angular meshwork in these animals.
机译：为了使用综合的蛋白质组织学方法来深入了解眼科动物的前节变化。对2岁和5岁的眼科和正常兔（n = 20）的眼睛进行了组织学研究。房水（AH）的液相色谱-串联质谱（LC-MS / MS）用于确定动物组之间的差异蛋白表达。对通过LC-MS / MS鉴定的差异表达蛋白进行蛋白质印迹和免疫组化分析。该眼科动物表现出轻度的临床表型，典型的角度异常表现出组织学上的进展。在所有强直性兔中，Descemet膜（DM）和前晶状体囊明显增厚，表明纤连蛋白和胶原IV免疫标记增加。采用严格过滤标准的LC-MS / MS在这些兔子中发现了几种AH蛋白的显着差异表达。 2岁组中的目标蛋白是富含组氨酸的糖蛋白，而5岁组中的蛋白包括α-2-HS-糖蛋白，簇蛋白，载脂蛋白E，感光受体类维生素A结合蛋白，运甲状腺素蛋白， Cochlin，凝溶胶蛋白，触珠蛋白，血红蛋白和β-2微球蛋白。通过蛋白质印迹和免疫组织化学验证所选蛋白质的蛋白质组学数据。改变的AH蛋白影响了广泛的官能团。这些包括细胞外基质的调节，细胞凋亡的调节，氧化应激和蛋白质转运。在组织学上，在眼科兔子中发现了多个前节改变，随着年龄的增长逐渐改变。在这些兔子中差异表达的AH蛋白表明AH在调节这些动物的DM，前晶状体囊和角网中的病理变化方面具有多功能作用。

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