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首页> 外文期刊>Transplant immunology >The immunological role of lipid transfer/metabolic proteins in liver transplantation tolerance.
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The immunological role of lipid transfer/metabolic proteins in liver transplantation tolerance.

机译：脂质转移/代谢蛋白在肝移植耐受中的免疫学作用。

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BACKGROUND: In a rat tolerogenic orthotopic liver transplantation (OLT) model, recipient serum after OLT (post-OLT serum) has been reported to prevent allograft rejection. A previous proteomic study indicated that apolipoprotein E (apo-E), which is an important factor for cholesterol transportation, is expressed at the latter tolerogenic phase after OLT. It has also been known that adipose tissue-derived adipokine, adiponectin, is an essential factor for fatty acid catabolism. This study aimed to characterize the role of lipid transfer/metabolic proteins in liver transplantation tolerance. METHODS: To identify the apo-E and adiponectin in post-OLT serum, Western analyses and enzyme-linked immunosorbent assay (ELISA) were performed, respectively. The immunosuppressive activities of those factors were evaluated by inhibition of the mixed lymphocyte reaction (MLR). RESULTS: Western analyses showed that the mobility of apo-E was shifted at the latter tolerogenic phase after OLT in a natural tolerance model, and a similar phenomenon was confirmed in the serum of a drug-induced tolerance model (rejection model+cyclosporin A (CsA); 0 to 14 days) after cessation of CsA. Further study revealed that neutralization of modified apo-E in post-OLT serum reduced the immunosuppressive activity. Additionally, plasma adiponectin was significantly elevated at the latter phase after OLT, and possessed MLR-inhibitory activity. CONCLUSIONS: These results suggest that the mobility shift of apo-E and/or the up-regulation of adiponectin may be necessary for overcoming the rejection, recovering the liver allograft function, and following tolerance induction in experimental OLT models, and may be useful as one indicator to surmise the prognosis after liver transplantation.
机译：背景：在大鼠耐受原位肝移植（OLT）模型中，据报道OLT后的受体血清（OLT后血清）可防止同种异体移植排斥。先前的蛋白质组学研究表明，载脂蛋白E（apo-E）是胆固醇运输的重要因素，在OLT后的致耐受期表达。还已知脂肪组织衍生的脂肪因子脂联素是脂肪酸分解代谢的必要因素。这项研究旨在表征脂质转移/代谢蛋白在肝移植耐受中的作用。方法：为鉴定OLT后血清中的apo-E和脂联素，分别进行了Western分析和酶联免疫吸附测定（ELISA）。这些因子的免疫抑制活性通过抑制混合淋巴细胞反应（MLR）来评估。结果：西方分析表明，在自然耐受模型中，apo-E的迁移率在OLT后的致耐受期发生了转移，并且在药物诱导的耐受模型的血清中也证实了类似现象（排斥模型+环孢菌素A（ CsA）;停止CsA后的0到14天）。进一步的研究表明，中和后OLT血清中修饰的apo-E会降低免疫抑制活性。此外，血浆脂联素在OLT后的后期显着升高，并具有MLR抑制活性。结论：这些结果表明，在实验性OLT模型中克服排斥反应，恢复肝脏同种异体移植功能和诱导耐受后，apo-E的迁移率的移动和/或脂联素的上调可能是必要的。评估肝移植后预后的一项指标。

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