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首页> 外文期刊>Transfusion: The Journal of the American Association of Blood Banks >Primitive human HPCs are better maintained and expanded in vitro at 1 percent oxygen than at 20 percent.
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Primitive human HPCs are better maintained and expanded in vitro at 1 percent oxygen than at 20 percent.

机译：原始人类HPC在20％的氧气条件下在体外能更好地维持和扩展。

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BACKGROUND: The liquid culture of murine bone marrow cells at 1-percent oxygen maintains the balance between primative progenitor cell renewal and clonogenic progenitor expansion better than that at 20-percent oxygen. These results are of potential interest for the ex vivo expansion of human progenitor cells, as low O(2) tension could preserve the engraftment potential of cultured apheresis products. STUDY DESIGN AND METHODS: G-CSF-mobilized blood cells collected by apheresis, now the main source of progenitor cells for autologous transplantation, were cultured at 1-percent and 20-percent O(2) for 7 days in serum-free liquid cultures in the presence of IL-3 and SCF (5 ng/mL). The growth of the clonogenic progenitors (CFU-GM, BFU-E, CFU-Mix) and of the more primitive human HPCs that are capable of generating clongenic progenitors in secondary liquid culture, as well as the proliferation and differentiation of total and CD34+ cells, was analyzed. RESULTS: The expansion of CD34+ cells and of clonogenic progenitors was significantly lower in liquid cultures at 1-percent O(2) than at 20-percent O(2). On the contrary, the primitive human HPCs were better maintained and expanded at 1-percent O(2), although the number of CD34+ cells remaining quiescent was lower. After 7 days of liquid culture at 1-percent or 20-percent O(2) the percentage of CD34+ cells was similar. However, the CD34+ cells that divided more than four times (PKH2 staining) were more numerous in liquid cultures incubated at 1-percent O(2). CONCLUSION: When cultured at 1-percent O(2) for 7 days in presence of IL-3 and SCF, the CD34+ cells present in apheresis components underwent more cell divisions and better maintained their primitive progenitor cell potential. As suggested by previous results in mice, our data on human cells emphasize the potential interest of cultures at low O(2) tension (1%) for cell therapy protocols aimed at expanding primitive HPCs in autografts.
机译：背景：在氧气浓度为1％时，鼠骨髓细胞的液体培养比在氧气浓度为20％时更好地维持了原始祖细胞更新和克隆形成祖细胞膨胀之间的平衡。这些结果对于人类祖细胞的体外扩增具有潜在的意义，因为低的O（2）张力可以保留培养的血液分离术产品的植入潜能。研究设计和方法：通过单采血液收集的G-CSF动员的血细胞（现在是自体移植的祖细胞的主要来源）在无血清液体培养物中分别以1％和20％O（2）培养7天。在IL-3和SCF（5 ng / mL）存在下。克隆形成祖细胞（CFU-GM，BFU-E，CFU-Mix）和更原始的人类HPC的生长，这些人类HPC能够在二次液体培养中生成克隆生成祖细胞，以及总细胞和CD34 +细胞的增殖和分化，进行了分析。结果：CD34 +细胞和克隆形成的祖细胞的扩增在液体培养物中在1％O（2）时显着低于20％O（2）。相反，原始的人类HPC可以更好地保持和扩展到1％的O（2），尽管保持静止的CD34 +细胞的数量更低。在1％或20％O（2）的液体培养7天后，CD34 +细胞的百分比相似。但是，分裂超过四倍的CD34 +细胞（PKH2染色）在以1％O（2）孵育的液体培养物中数量更多。结论：当在IL-3和SCF存在下于1％O（2）下培养7天时，单采血液成分中存在的CD34 +细胞经历了更多的细胞分裂，并更好地保持了其原始祖细胞的潜能。正如先前在小鼠中的结果所表明的那样，我们在人类细胞上的数据强调了在低O（2）张力（1％）的条件下培养物的潜在兴趣，这些疗法旨在扩大自体移植中的原始HPC。

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