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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Isolation of Arcanobacterium (Actinomyces) pyogenes from Abscessed Cattle Kidney and Identification by PCR
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Isolation of Arcanobacterium (Actinomyces) pyogenes from Abscessed Cattle Kidney and Identification by PCR

机译：牛脓肾中化脓性产弧菌（放线菌）的分离及PCR鉴定

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摘要

In this study, the presence of Arcanobacterium spp., which causes a variety of purulent infections involving the skin, joints and visceral organs, was investigated in abscessed kidney samples of cattle, A total of 500 cattle were examined at postmortem and 100 samples with abscess were collected. Arcanobacterium pyogenes was isolated in 40 (40%) of the samples examined DNA extracted from the isolates were amplified by Polymerase Chain Reaction (PCR) using specific primers derived from pio gene of A.pyogenes and all the isolates were determined to be positive by PCR. It was concluded that PCR employed in this study may be applied for identification of A, pyogenes.
机译：在这项研究中，对牛脓肿的肾脏样本中存在导致多种皮肤，关节和内脏器官化脓性感染的Arcanobacterium spp。的存在进行了调查，死后的肾脏样本中共检查了500头牛，脓肿样本中有100头被收集。在40个（40％）的受检样品中分离出化脓性弧菌（Arcanobacterium pyogenes），使用聚合酶链球菌（A.pyogenes）pio基因衍生的特异引物，通过聚合酶链反应（PCR）扩增从分离物中提取的DNA，并通过PCR确定所有分离物均为阳性。结论是，该研究中采用的PCR可用于鉴定化脓性链球菌。

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