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New dimensions for VIGS in plant functional genomics

机译:植物功能基因组学中VIGS的新领域

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摘要

Virus-induced gene silencing (VIGS) is an efficient tool for gene function studies. It has been used to perform both forward and reverse genetics to identify plant genes involved in several plant processes. However, this technology has not yet been used to its full potential. This can be attributed to several of its limitations such as inability to silence genes during seed germination and the non-stable nature of silencing. However, several recent studies have shown that these limitations can now be overcome. In this review, we will discuss the limitations of VIGS and suitable solutions. In addition, we also describe the recent improvements and future prospects of using VIGS in plant biology.
机译:病毒诱导的基因沉默(VIGS)是进行基因功能研究的有效工具。它已被用于执行正向和反向遗传学,以鉴定涉及几种植物过程的植物基因。但是,这项技术尚未充分发挥其潜力。这可以归因于它的几个局限性,例如在种子发芽过程中无法沉默基因以及沉默的不稳定性质。但是,最近的一些研究表明,现在可以克服这些限制。在这篇评论中,我们将讨论VIGS的局限性和合适的解决方案。此外,我们还描述了在植物生物学中使用VIGS的最新改进和未来前景。

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