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Systems analysis of metabolic phenotypes: what have we learnt?

机译:代谢表型的系统分析:我们学到了什么?

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Flux is one of the most informative measures of metabolic behavior. Its estimation requires integration of experimental and modeling approaches and, thus, is at the heart of metabolic systems biology. In this review, we argue that flux analysis and modeling of a range of plant systems points to the importance of the supply of metabolic inputs and demand for metabolic end-products as key drivers of metabolic behavior. This has implications for metabolic engineering, and the use of in silico models will be important to help design more effective engineering strategies. We also consider the importance of cell type-specific metabolism and the challenges of characterizing metabolism at this resolution. A combination of new measurement technologies and modeling approaches is bringing us closer to integrating metabolic behavior with whole-plant physiology and growth.
机译:助焊剂是代谢行为最有用的指标之一。其估计需要整合实验和建模方法,因此是代谢系统生物学的核心。在这篇综述中,我们认为一系列植物系统的通量分析和建模指出了代谢输入的供应和对代谢终产物的需求作为代谢行为的关键驱动因素的重要性。这对代谢工程具有影响,计算机模型的使用对于帮助设计更有效的工程策略将非常重要。我们还考虑了特定于细胞类型的代谢的重要性以及以这种分辨率表征代谢的挑战。新的测量技术和建模方法的结合使我们更接近于将代谢行为与全株生理和生长相结合。

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