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首页> 外文期刊>Transplantation Proceedings >Bloodstream cells phenotypically identical to human mesenchymal bone marrow stem cells circulate in large amounts under the influence of acute large skin damage: new evidence for their use in regenerative medicine.
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Bloodstream cells phenotypically identical to human mesenchymal bone marrow stem cells circulate in large amounts under the influence of acute large skin damage: new evidence for their use in regenerative medicine.

机译：在急性大面积皮肤损伤的影响下，与人间充质干细胞在表型上相同的血细胞大量循环：它们在再生医学中的应用的新证据。

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OBJECTIVES: Recent work has shown that human bone marrow contains mesenchymal stem cells (MSCs). However, little is known about their presence in peripheral blood. Since these cells are potentially responsible for tissue repair after injury, their number should be increased during these situations. To demonstrate their number during these situations, we measured MSCs in the peripheral blood of healthy donors and burn patients. MATERIALS AND METHODS: Blood samples were obtained from 15 acute burn patients and 15 healthy donors. We performed flow cytometric analysis, using a large monoclonal antibody panel: CD44, CD45, CD14, DR, CD34, CD19, CD13, CD29, CD105, CD1a, CD90, CD38, CD25. MSC phenotype was considered positive for CD44, CD13, CD29, CD90, and CD105, and negative for the other monoclonals. The testing was performed on day 3 after injury. We correlated the results with the age, sex, and size and type of burns. RESULTS: Cells expressing the MSC phenotype were detected in the peripheral blood of both groups. Noteworthy, compared with samples from healthy donors (0.0078 +/- 0.0044), blood obtained from burn patients showed a higher MSC percentage (0.1643 +/- 0.115; P < .001). The percentage of MSCs correlated with the size and severity of the burn. Increased values were also observed among younger patients. CONCLUSIONS: MSCs have an important role in regenerative processes of human tissues. We found cells phenotypically identical to MSCs circulating in physiological number in normal subjects, but in significantly higher amounts during acute large burns. Therefore, they may represent a previously unrecognized circulatory component to the process of skin regeneration.
机译：目的：最近的研究表明，人骨髓中含有间充质干细胞（MSCs）。然而，关于它们在外周血中的存在知之甚少。由于这些细胞可能在损伤后负责组织修复，因此在这些情况下应增加它们的数量。为了证明在这种情况下的数量，我们测量了健康供体和烧伤患者外周血中的MSC。材料与方法：血液样本取自15例急性烧伤患者和15例健康供体。我们使用大型单克隆抗体组进行了流式细胞仪分析：CD44，CD45，CD14，DR，CD34，CD19，CD13，CD29，CD105，CD1a，CD90，CD38，CD25。 MSC表型被认为对CD44，CD13，CD29，CD90和CD105呈阳性，而对其他单克隆呈阴性。受伤后第3天进行测试。我们将结果与年龄，性别，烧伤的大小和类型相关联。结果：在两组外周血中均检测到表达MSC表型的细胞。值得注意的是，与健康捐赠者的样本（0.0078 +/- 0.0044）相比，烧伤患者的血液显示出更高的MSC百分比（0.1643 +/- 0.115; P <.001）。 MSC的百分比与烧伤的大小和严重程度相关。在年轻患者中也观察到数值增加。结论：间充质干细胞在人体组织再生过程中具有重要作用。我们发现在正常受试者中，与生理循环中的MSC循环细胞在表型上相同，但是在急性大面积烧伤中其数量明显更高。因此，它们可能代表了皮肤再生过程中以前无法识别的循环成分。

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