Cultivating the uncultivated: a community genomics perspective

Tyson GW; Banfield JF

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Cultivating the uncultivated: a community genomics perspective

机译：培育无人耕种：社区基因组学的观点

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Current isolation methods access only a small subset of the total microbial diversity. Although an isolate traditionally has been required for genomic characterization, the advent of sequencing of entire natural microbial communities enables culture-independent genomic analysis. Information about the genetic potential of uncultivated organisms can be used to predict the form of metabolic interdependencies and nutritional requirements. We believe that this could provide the information necessary to bypass bottlenecks that have inhibited cultivation of many microorganisms. However, it might not be practical or possible to isolate all of the vast number of microbial species and strains for laboratory-based characterization. Ultimately, cultivation-independent genomic and genomically enabled approaches could provide a way to directly analyze microbial activity in its geochemical and ecological context.

机译：当前的隔离方法仅访问总微生物多样性的一小部分。尽管传统上需要分离株来进行基因组表征，但是对整个天然微生物群落进行测序的出现使得能够进行与培养物无关的基因组分析。有关未培养生物遗传潜力的信息可用于预测代谢相互依存和营养需求的形式。我们相信，这可以提供必要的信息，以绕过抑制许多微生物培养的瓶颈。但是，分离所有大量的微生物物种和菌株以进行基于实验室的表征可能不切实际或不可行。最终，不依赖栽培的基因组学和基因组学方法可以提供一种直接分析其地球化学和生态环境中微生物活动的方法。

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《Trends in Microbiology》 |2005年第9期|共5页
作者
Tyson GW; Banfield JF;
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原文格式 PDF
正文语种 eng
中图分类微生物学;
关键词
Bacteria genetics growth amp; development isolation amp; purification; Ecosystem; Environmental Microbiology; Genomics;

机译：细菌遗传学的生长与发育分离与纯化;生态系统;环境微生物学;基因组学;

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1. Cultivating the uncultivated: a community genomics perspective [J] . Tyson GW, Banfield JF Trends in Microbiology . 2005,第9期

机译：培育无人耕种：社区基因组学的观点
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3. Cultivating Contemporary Chinese People’s Global Consciousness from the Perspective of a Community with a Shared Future for Mankind [J] . Yuwen Li, Xu Qingtong(译) 当代社会科学（英文） . 2019,第001期

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4. REVIEW: Genomic approaches for characterising and quantifying microbial communities to the benefit of the industry: An environmental perspective [C] . Biennial Conference of the Australasian^Pig^Science^Association . 2013

机译：回顾：基因组方法，用于表征和量化微生物社区的利益：环境视角
5. The uncultivated vascular flora and plant communities of Powell Botanical Gardens. [D] . Harris, Alex W. 2010

机译：鲍威尔植物园未栽培的维管束和植物群落。
6. Genomic characteristics and environmental distributions of the uncultivated Far-T4 phages [O] . Simon Roux, François Enault, Viviane Ravet, 2020

机译：未培养的Far-T4噬菌体的基因组特征和环境分布
7. Draft Council directive concerning freedom of establishment on farms that have been derelict or uncultivated for more than two years. Draft Council directive on freedom of establishment in agriculture for nationals of a Member State who have worked as paid agricultural workers in another Member State for an unbroken period of two years. Draft Council directive for the removal of restrictions on travel and residence by nationals of Member States within the Community as they affect freedom of establishment and freedom to supply services. Draft Council directive for co-ordination of the special measures governing the entry and residence of foreign nationals, where such measures are warranted on grounds of public policy, public safety or public health (Article 50). Draft Council directive for the removal of restrictions on freedom of establishment and freedom to supply services in reinsurance and retrocession. Draft Council directive on freedom of establishment and freedom to supply services in the form of activities serving trade and industry. Draft Council directive on freedom of establishment and freedom to supply services in connection with wholesale trade. Draft Council directive removing all prohibitions on or other obstacles to payment for services where payment restrictions are the only limiting factor on the supply. Draft Council first directive concerning cinematography. Supplement to Bulletin of the European Economic Community No. 9/10, 1962 [O] . 1962

机译：关于在废弃或未开垦两年以上的农场建立自由的理事会指令草案。理事会关于一个成员国国民的农业设立自由的指令草案，该成员国在另一个成员国作为有偿农业工人工作，为期两年。理事会关于取消成员国国民在共同体内旅行和居住限制的指令草案，因为它们影响到建立自由和提供服务的自由。关于协调外国人入境和居住的特别措施的理事会指令草案，其中有必要以公共政策，公共安全或公共卫生为由采取这些措施（第50条）。理事会关于取消对再保险和再保险的设立自由和提供服务自由限制的指令。理事会关于以贸易和工业服务活动形式提供自由和提供服务自由的指示。理事会关于建立自由和提供与批发贸易有关的服务自由的指令草案。理事会指令草案取消了对服务付款的所有禁令或其他障碍，其中支付限制是供应的唯一限制因素。理事会关于电影摄影的第一项指令草案。 1962年第9/10号欧洲经济共同体公报补编

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