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Microbial nanoscopy: a closer look at microbial cell surfaces

机译:微生物纳米检查:仔细观察微生物细胞表面

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How cell envelope constituents are spatially organised and how they interact with the environment are key questions in microbiology. Unlike other bioimaging tools, atomic force microscopy (AFM) provides information about the nanoscale surface architecture of living cells and about the localization and interactions of their individual constituents. These past years have witnessed remarkable advances in our use of the AFM molecular toolbox to observe and force probe microbial cells. Recent milestones include the real-time imaging of the nanoscale organization of cell walls, the quantification of subcellular chemical heterogeneities, the mapping and functional analysis of individual cell wall constituents and the analysis of the mechanical properties of single receptors and sensors.
机译:细胞包膜成分如何在空间上组织以及它们如何与环境相互作用是微生物学中的关键问题。与其他生物成像工具不同,原子力显微镜(AFM)提供有关活细胞纳米级表面结构以及其各个成分的定位和相互作用的信息。在过去的几年中,我们在使用AFM分子工具箱观察和强迫探测微生物细胞方面取得了显着进步。最近的里程碑包括细胞壁纳米级组织的实时成像,亚细胞化学异质性的定量,单个细胞壁成分的作图和功能分析以及单个受体和传感器的机械性能分析。

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