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What do microarrays really tell us about M. tuberculosis?

机译:微阵列真正告诉我们有关结核分枝杆菌的哪些信息?

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摘要

Bacterial pathogens adapt to their host environments to a large extent through switching on complex transcriptional programmes, and whole-genome microarray experiments promise to reveal this complexity. There has been a recent burst of articles reporting transcriptome analyses of Mycobacterium tuberculosis, including for the first time studies in macrophages and mice. We review gene expression reports, and compare them with each other and with microarray-based gene essentiality studies, revealing at times a startling lack of correlation. Additionally, we suggest a standardization format for the submission of processed data for publication, to facilitate cross-experiment analyses.
机译:细菌病原体通过启动复杂的转录程序而在很大程度上适应宿主环境,全基因组微阵列实验有望揭示这种复杂性。最近有大量报道结核分枝杆菌转录组分析的文章,包括首次在巨噬细胞和小鼠中进行的研究。我们审查了基因表达报告,并将它们与基于微阵列的基因本质研究进行了相互比较,发现有时缺乏惊人的相关性。此外,我们建议用于处理数据提交以供发布的标准化格式,以方便进行跨实验分析。

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