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The implications of a low rate of horizontal transfer in Borrelia

机译:博雷利亚水平转移率低的影响

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The nature and rate of recombination can be studied by comparing the sequences of multiple genes across a set of strains. When this approach is applied to Borrelia burgdorferi, four results emerge: (1) chromosomal genes are clonal; (2) there is little or no plasmid exchange; (3) the major mode of horizontal transfer of genetic material inserts a small fragment of DNA, typically <1 kb, during recombination; and (4) the level of horizontal transfer in Borrelia is so low that there is evidence for horizontal transfer only in genes where there is positive selection for diversity, that is, positive selection for the recombinant. Thus, Borrelia can serve as a model of a low recombination taxon. The implications of these results lead us to postulate thatan unknown agent that is part of the Borrelia genome mediates the horizontal transfer of small fragments of DNA; the rare transfer of small fragments of DNA excludes both DNA parasites and virulence factors from the genome.
机译:通过比较一组菌株中多个基因的序列,可以研究重组的性质和速率。当这种方法应用于伯氏疏螺旋体时,出现了四个结果:(1)染色体基因是克隆的; (2)很少或没有质粒交换; (3)在重组过程中,遗传物质水平转移的主要方式是插入一小段DNA片段,通常<1 kb; (4)疏螺旋体中水平转移的水平很低,以至于只有在对多样性具有正选择的基因中,即重组体的正选择,才有水平转移的证据。因此,疏螺旋体可以作为低重组分类群的模型。这些结果的含义使我们推测,一种未知的病原体是疏螺旋体基因组的一部分,它介导了DNA小片段的水平转移。 DNA小片段的罕见转移将基因组中的DNA寄生虫和毒力因子排除在外。

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