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Post-vaccination antibodies profile against Peste des petits ruminants (PPR) virus in sheep and goats of Punjab, Pakistan

机译:巴基斯坦旁遮普邦绵羊和山羊抗小反刍兽疫病毒(PPR)的疫苗接种后抗体概况

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A total of 70 sheep and 330 goats were selected randomly. All the animals were kept under same housing and management conditions. Serum samples were collected from all the animals and tested for the presence of antibodies against Peste des petits ruminants (PPR) virus using competitive ELISA (cELISA). All the animals were found negative showing percentage inhibition (PI) values <50. The animals were vaccinated against PPR with Nig/75/1 strain vaccine of PPR Serum samples were collected from randomly selected 12 sheep and 30 goats at 10, 30 and 45 days post-vaccination. The samples were subjected to cELISA to determine the presence of antibodies against PPRV. The samples with PI >50 were considered as sero-positive. The sheep found positive at 10, 30 and 45 days post-vaccination were 1(8.3%), 7(58.3%) and 12(100%) respectively. In case of goats 3(10.0%), 29(96.6%) and 27(90.0%) animals gave positive results at 10, 30 and 45 days post-vaccination respectively. Mean PI values in sheep at 10, 30 and 45 days post-vaccination were recorded as 37, 65 and 91 respectively, whereas in goats these values were 43, 78 and 86 respectively.
机译:随机选择了70只绵羊和330只山羊。所有动物都在相同的饲养和管理条件下饲养。从所有动物收集血清样品,并使用竞争性ELISA(cELISA)测试抗小反刍兽疫(PPR)病毒的抗体的存在。发现所有动物均为阴性,显示抑制百分率(PI)值<50。用PPR的Nig / 75/1株疫苗对动物接种PPR疫苗后10、30和45天从随机选择的12只绵羊和30只山羊中收集血清样品。将样品进行cELISA,以确定抗PPRV的抗体的存在。 PI> 50的样品被认为是血清阳性的。接种后第10、30和45天发现阳性的绵羊分别为1(8.3%),7(58.3%)和12(100%)。对于山羊,有3只(10.0%),29只(96.6%)和27只(90.0%)的动物分别在接种后10、30和45天获得了阳性结果。接种后10、30和45天,绵羊的平均PI值分别记录为37、65和91,而山羊则分别为43、78和86。

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