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首页> 外文期刊>Tropical Animal Health and Production >Evidence of Schmallenberg virus circulation in ruminants in Greece.
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Evidence of Schmallenberg virus circulation in ruminants in Greece.

机译：Schmallenberg病毒在希腊反刍动物中传播的证据。

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During March 2013, we investigated the presence and the levels of Schmallenberg virus (SBV) circulation in three dairy cow herds and three sheep flocks in Central Macedonia, Greece. In two cow herds, a high number of abortions had been observed during the winter. Six bulk-tank milk samples and 147 individual sera were screened for SBV-specific antibodies by ELISA. Positive reactions were obtained from 5 out of 6 bulk-tank milk samples, 58 out of 90 sera from the 3 cow herds, and 2 sera from 2 of the 3 sheep flocks. Twenty-two ELISA-positive sera were tested by serum neutralization test (SNT). SNT confirmed the presence of neutralizing antibodies against SBV in all samples tested, with titers ranging between 1:32 and >=1:256. No neutralizing antibodies against Akabane virus (AKAV) or Shamonda virus (SHAV) were detected, indicating that neutralizing antibodies against SBV do not cross react with AKAV or SHAV in SNT. ELISA testing of bulk-tank milk samples proved to be convenient and reliable. None of the tested sera was found positive for SBV by real-time RT-PCR, indicating that the sampling was conducted past the viremia stage. This is the first report of SBV circulation in Greece.
机译：2013年3月，我们调查了希腊中马其顿的三只奶牛群和三只羊群中Schmallenberg病毒（SBV）的存在和水平。在冬季，在两个牛群中观察到大量流产。通过ELISA对SBV特异性抗体筛查了六个散装罐装牛奶样品和147个单独的血清。从6个散装罐装牛奶样品中的5个，3个牛群的90个血清中的58个，以及3个羊群中的2个血清中的2个血清中获得了阳性反应。通过血清中和试验（SNT）检测了22个ELISA阳性血清。 SNT确认所有测试样品中均存在针对SBV的中和抗体，效价范围为1:32至> = 1：256。没有检测到针对赤羽病毒（AKAV）或Shamonda病毒（SHAV）的中和抗体，表明针对SBV的中和抗体不会与SNT中的AKAV或SHAV交叉反应。散装牛奶样品的ELISA测试被证明是方便和可靠的。实时RT-PCR未发现任何被测血清对SBV呈阳性，表明采样是在病毒血症阶段之后进行的。这是希腊SBV发行的第一份报告。

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