• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Tropical Animal Health and Production >Expression profile of HSP genes during different seasons in goats (Capra hircus)
【24h】

Expression profile of HSP genes during different seasons in goats (Capra hircus)

机译：山羊（Capra hircus）不同季节HSP基因的表达谱

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The present study has demonstrated the expression of HSP60, HSP70, HSP90, and UBQ in peripheral blood mononuclear cells (PBMCs) during different seasons in three different age groups (Groups I, II, and III with age of 0-2, 2-5, and >5 years, respectively) of goats of tropical and temperate regions. Real-time polymerase chain reaction was applied to investigate mRNA expression of examined factors. Specificity of the desired products was documented using analysis of the melting temperature and high-resolution gel electrophoresis to verify that the transcripts are of the exact molecular size predicted. The mRNA expression of HSP60, HSP90, and UBQ was significantly higher (P<0.05) in all age groups during peak summer season as compared with peak winter season in both tropical and temperate region goats. HSP70 mRNA expression was significantly higher (P<0.05) during summer season as compared with winter season in tropical region goats. However, in the temperate region, in goats from all the three age groups studied, a non-significant difference of HSP70 expression between summer and winter seasons was noticed. In conclusion, results demonstrate that (1) HSP genes are expressed in caprine PBMCs and (2) higher expression of HSPs during thermal stress suggest possible involvement of them to ameliorate deleterious effect of thermal stress so as to maintain cellular integrity and homeostasis in goats.Digital Object Identifier http://dx.doi.org/10.1007/s11250-012-0155-8
机译：本研究证明了三个不同年龄组（I，II和III组，年龄分别为0-2、2-5）在不同季节中，外周血单个核细胞（PBMC）中HSP60，HSP70，HSP90和UBQ的表达，和> 5年）的热带和温带地区的山羊。实时聚合酶链反应用于调查所审查的因素的mRNA表达。使用解链温度分析和高分辨率凝胶电泳记录所需产物的特异性，以验证转录本具有预测的确切分子大小。在热带和温带地区的山羊中，与冬季的峰值相比，在夏季的峰值季节，所有年龄组的HSP60，HSP90和UBQ的mRNA表达均显着较高（P <0.05）。在热带地区山羊中，与冬季相比，夏季HSP70 mRNA表达明显高于冬季（P <0.05）。然而，在温带地区，在所研究的所有三个年龄组的山羊中，注意到夏季和冬季之间HSP70表达的差异不显着。总之，结果表明：（1）HSP基因在热应激过程中在山羊PBMC中表达，（2）HSP的较高表达表明它们可能参与改善热应激的有害作用，从而维持山羊的细胞完整性和体内稳态。数字对象标识符http://dx.doi.org/10.1007/s11250-012-0155-8

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号