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首页> 外文期刊>Tropical Animal Health and Production >Molecular detection of bovine immunodeficiency virus in water buffaloes (Bubalus bubalis) from the Amazon region, Brazil
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Molecular detection of bovine immunodeficiency virus in water buffaloes (Bubalus bubalis) from the Amazon region, Brazil

机译：巴西亚马逊地区水牛（Bubalus bubalis）中牛免疫缺陷病毒的分子检测

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摘要

Bovine immunodeficiency is a chronic progressive disease caused by a lentivirus that affects cattle and buffaloes. Although the infection has been described in cattle in some countries, including in Brazil, there are only two reports of infection in buffaloes: one in Pakistan and one in Cambodia. The aim of the present study was to survey the occurrence of bovine immunodeficiency virus (BIV) in water buffaloes from the Amazon region, Para state, Brazil. BIV proviral DNA was surveyed in 607 whole blood samples of water buffaloes from 10 farms located in the state of Para using semi-nested polymerase chain reaction (PCR) (PCR-SN) to amplify the pol region of the viral genome. Of the 607 samples tested, 27 (4.4 %) were positive for BIV proviral DNA. The amplified fragments were confirmed by sequence analysis after cloning and nucleotide sequencing. The sequence obtained had 99 % similarity to the reference strain (R-29). The present study provides important epidemiological data because BIV was detected for the first time in water buffaloes in Brazil. Further, the results suggest the possibility of the virus being a risk factor for herd health because it may be a potential causal agent of chronic disease and, also may be associated to other infectious diseases.
机译：牛免疫缺陷是由慢病毒引起的一种慢性进行性疾病，会影响牛和水牛。尽管已经在包括巴西在内的一些国家中描述了牛感染的情况，但只有两份关于水牛感染的报道：一个在巴基斯坦，一个在柬埔寨。本研究的目的是调查巴西帕拉州亚马逊地区水牛中牛免疫缺陷病毒（BIV）的发生情况。使用半巢式聚合酶链反应（PCR）（PCR-SN）对来自帕拉州10个农场的水牛的607份全血样本中的BIV前病毒DNA进行了调查，以扩增病毒基因组的pol区域。在607个样本中，有27个（4.4％）BIV前病毒DNA呈阳性。克隆和核苷酸测序后，通过序列分析确认扩增的片段。获得的序列与参考菌株（R-29）具有99％的相似性。由于首次在巴西的水牛中检测到BIV，因此本研究提供了重要的流行病学数据。此外，结果表明该病毒可能是畜群健康的危险因素，因为它可能是慢性疾病的潜在病原体，也可能与其他传染病有关。

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