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Assessing the effectiveness of intubation as a challenge model in contagious bovine pleuropneumonia vaccine experiments

机译：在传染性牛胸膜肺炎疫苗实验中评估插管作为挑战模型的有效性

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A study was carried out to assess the effectiveness of a bronchoscope in administering a pathogenic field strain of Mycoplasma mycoides subsp. mycoides (MmmSC) in cattle challenge experiments. Out of 16 animals inoculated using the bronchoscope, 10 (62.2%) showed clinical disease as evidenced by fever and 15 (93.8%) displayed typical lesions of CBPP from which MmmSC was isolated. Serum samples collected weekly were tested by Complement Fixation Test (CFT) and competitive enzyme-linked immunosorbent assay (c-ELISA). Antibodies to MmmSC were detected in 10 out of the 16 animals by the CFT and 11 out of the 16 animals by c-ELISA. The onset of clinical disease was as early as 2 days post-inoculation, and most of the animals developed clinical disease 2 to 3 weeks post-infection. These results clearly demonstrate that nasotracheal inoculation of pathogenic strain of MmmSC with the aid of a bronchoscope can lead to early onset of clinical disease; similar to previous studies but with higher numbers of animals showing clinical disease. This is in contrast with previous studies where early clinical disease was observed in as little as 15% of inoculated animals. This nasotracheal inoculation method using a bronchoscope can, therefore, be adopted for use in experimental challenge infections of cattle. This method is found to be a better replacement to the contact transmission method whose drawback includes extra cost of donor animals and unpredictable rate and timing of transmission from intubated to challenge animals.
机译：进行了一项研究，以评估支气管镜在施用支原体支原体亚种的致病性田间菌株中的有效性。牛激发实验中的杀菌剂（MmmSC）。在用支气管镜接种的16只动物中，有10例（62.2％）表现出临床症状，如发烧所证明，还有15例（93.8％）表现出典型的CBPP病变，可从中分离出MmmSC。每周收集的血清样品通过补体固定测试（CFT）和竞争性酶联免疫吸附测定（c-ELISA）进行测试。通过CFT在16只动物中的10只中检测到MmmSC抗体，通过c-ELISA在16只动物中检测到11只。临床疾病的发作最早在接种后2天，大多数动物在感染后2至3周发展为临床疾病。这些结果清楚地表明，在支气管镜的辅助下气管内接种MmmSC致病株可导致临床疾病的早发。与先前的研究相似，但有更多的动物表现出临床疾病。这与先前的研究相反，在先前的研究中，仅15％的被接种动物就观察到了早期临床疾病。因此，可以将这种使用支气管镜的鼻气管接种方法用于牛的实验性感染。发现该方法是接触传播方法的更好的替代，接触传播方法的缺点包括供体动物的额外成本以及从插管动物向挑战动物传播的不可预测的速率和时机。

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