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首页> 外文期刊>Tropical Animal Health and Production >Prevalence of porcine circovirus-2 DNA-positive ovarian and uterine tissues in gilts culled due to reproductive disturbance in Thailand
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Prevalence of porcine circovirus-2 DNA-positive ovarian and uterine tissues in gilts culled due to reproductive disturbance in Thailand

机译：在泰国由于繁殖障碍而被淘汰的猪圆环病毒2 DNA阳性卵巢和子宫组织的患病率

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摘要

The present study aimed to determine the prevalence of porcine circovirus-2 (PCV-2) DNA-positive ovarian and uterine tissues in gilts culled due to reproductive disturbance in Thailand. Tissues (70 ovaries and 102 uteri) and serum (n = 102) samples from 102 gilts were included. PCV-2 DNA was detected by using polymerase chain reactions. The localisation of PCV-2 antigen was determined by immunohistochemistry, and PCV-2 antibody was evaluated by ELISA. PCV-2 DNA was detected in 30.0 % (21/70) of the ovaries and in 45.1 % (46/102) of the uteri. Age did not influence the frequency of PCV-2 DNA detection in these reproductive organs of gilts (P > 0.05). The prevalence of PCV-2 DNA-positive uterine tissue in gilts culled due to non-reproductive problems (20.0 %) was lower than gilts culled due to abortion (85.0 %), abnormal vaginal discharge (47.5 %) and anoestrus (53.5 %) (P 0.05). The prevalence of PCV-2 DNA-positive uterine tissue in the gilts with high antibody titres (23.0 %) was lower than in gilts with low antibody titres (57.6 %) and seronegative gilts (64.5 %) (P 0.05). PCV-2 immunostaining was detected in the endometrial cells, lymphocytes and macrophages of the uteri and in oocytes and granulosa cells of the ovaries. In conclusion, the detection of PCV-2 in the reproductive organs reveals an important potential impact of this virus on the reproductive apparatus in gilts.
机译：本研究旨在确定在泰国因繁殖障碍而被淘汰的后备母猪的猪圆环病毒2（PCV-2）DNA阳性卵巢和子宫组织的患病率。包括来自102个小母猪的组织（70个卵巢和102个子宫）和血清（n = 102）样本。通过使用聚合酶链反应检测PCV-2 DNA。通过免疫组织化学确定PCV-2抗原的定位，并通过ELISA评估PCV-2抗体。在30.0％（21/70）的卵巢和45.1％（46/102）的子宫中检测到PCV-2 DNA。年龄并未影响这些小母猪生殖器官中PCV-2 DNA的检出频率（P> 0.05）。因非生殖问题而被淘汰的小母猪中PCV-2 DNA阳性子宫组织的患病率（20.0％）低于因流产而被淘汰的小母猪（85.0％），异常阴道分泌物（47.5％）和性发情（53.5％） （P ＜0.05）。高抗体滴度的后备母猪（23.0％）的PCV-2 DNA阳性子宫组织的患病率低于低抗体滴度的后备母猪（57.6％）和血清阴性的后备母猪的患病率（64.5％）（P <0.05）。在子宫内膜细胞，淋巴细胞和巨噬细胞以及卵巢的卵母细胞和颗粒细胞中检测到PCV-2免疫染色。总之，在生殖器官中检测到PCV-2揭示了该病毒对后备母猪生殖器的重要潜在影响。

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