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首页> 外文期刊>Turkish Journal of Fisheries & Aquatic Sciences >Determination of Genetic Variations of Five Different Indigenous Trout Species in Turkey (Salmo trutta sp L.) by Microsatellite Markers
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Determination of Genetic Variations of Five Different Indigenous Trout Species in Turkey (Salmo trutta sp L.) by Microsatellite Markers

机译：利用微卫星标记确定土耳其（Salmo trutta sp L.）五个特质鳟鱼的遗传变异

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摘要

In this study, the genotypic differences of five different trout populations were compared using microsatellite markers. Fish analyzed were; Balikli Lake (BLK) and Abant Lake (ABN) found in lakes, and Firat Basin (FRT), Aras Basin (ARS), oruh Basin (CRH) found in rivers. Allele numbers in each locus were determined by application of Single Strand Conformation Polymorphism (SSCP) to the microsatellite sections. Totally 62 alleles were observed from 10 microsatellite loci. According to the results from factorial correspondence analysis, it was determined that all of the populations formed different groups from each other. Considering all loci, observed heterozygosity (H-o) and expected heterozygosity (He), values changed from 0.602-0.668 and 0.570-0.670, respectively. Ho value was significantly (P<0.01) higher than He value of populations of FRT. Fixation index for subpopulations (F-IS) values were ranged from 0.069 to -0.173 (BLK to FRT) in populations, and the overall average diversity between populations (F-ST) value of the all loci was calculated as 0.0790. According to FST values calculated among populations, genetic distance was found to be significant (P<0.05) among four populations. It was determined that four of five populations were at the Hardy-Weinberg equilibrium (HWE). Considering genetic distance in populations, it was observed that the distance was shorter in lake forms compared to River forms.
机译：在这项研究中，使用微卫星标记比较了五个不同鳟鱼种群的基因型差异。分析的鱼是;在湖泊中发现了Balikli湖（BLK）和Abant湖（ABN），在河流中发现了Firat盆地（FRT），Aras盆地（ARS）和oruh盆地（CRH）。通过将单链构象多态性（SSCP）应用于微卫星切片，确定每个基因座中的等位基因数目。从10个微卫星基因座中总共观察到62个等位基因。根据阶乘对应分析的结果，可以确定所有人口都形成了不同的群体。考虑所有基因座，观察到的杂合度（H-o）和预期杂合度（He），其值分别从0.602-0.668和0.570-0.670改变。 Ho值显着高于PRT人群的He值（P <0.01）。群体中亚群固定值（F-IS）值的范围从0.069到-0.173（BLK到FRT），所有基因座的总体总体平均多样性（F-ST）值计算为0.0790。根据各种群间的FST值，发现四个种群之间的遗传距离显着（P <0.05）。确定五个人口中有四个处于Hardy-Weinberg平衡（HWE）。考虑到种群中的遗传距离，观察到湖泊形式的距离比河流形式的距离短。

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