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首页> 外文期刊>Carbohydrate research >An enzymatic method for preparation of homopolymannuronate blocks and strictly alternating sequences of mannuronic and guluronic units
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An enzymatic method for preparation of homopolymannuronate blocks and strictly alternating sequences of mannuronic and guluronic units

机译：一种酶法制备甘露糖醛酸和古洛糖醛酸单元的均聚甘露糖醛酸酯嵌段和严格交替的序列

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摘要

Two Pseudomonas aeruginosa alginates were lysed by an overexpressed polymannuronate lyase AlxM(B) (only acting on two or more consecutive, nonacetylated mannuronate units) to prepare either mannuronate blocks (poly-M blocks) with dp similar to 30, or strictly alternating sequences of mannuronic and guluronic acid (poly-MG blocks) with dp > 20. The poly-M blocks were obtained by lysis of a P. aeruginosa polymannuronate that has 50% O-acetylation at C-2 and C-3. The poly-MG blocks were obtained from a P. aeruginosa alginate that contained both mannuronate and guluronate residues. The polysaccharide was first deacetylated and then treated with the lyase to excise the mannuronate units from the alternating-MG blocks. Both types of blocks should have potent biological effects and should provide useful specific substrates for characterisation of other alginate lyases. (C) 1998 Elsevier Science Ltd. All rights reserved. [References: 31]
机译：用过量表达的多甘露糖醛酸裂解酶AlxM（B）（仅作用于两个或多个连续的非乙酰化甘露糖醛酸单位）裂解两个铜绿假单胞菌藻酸盐，以制备dp类似于30的甘露糖醛酸酯嵌段（poly-M嵌段），或严格交替的序列dp> 20的甘露糖醛酸和古洛糖醛酸（聚MG嵌段）。聚M嵌段是通过裂解在C-2和C-3处具有50％O-乙酰化的铜绿假单胞菌多甘露糖酸酯获得的。聚MG嵌段得自铜绿假单胞菌藻酸盐，其含有甘露糖醛酸酯和古洛糖酸酯残基。首先将多糖脱乙酰化，然后用裂解酶处理，以从交替的MG嵌段中切出甘露糖酸酯单元。两种类型的嵌段均应具有有效的生物学作用，并应提供有用的特异性底物来表征其他藻酸盐裂解酶。 （C）1998 Elsevier ScienceLtd。保留所有权利。 [参考：31]

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