• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Veterinary Clinical Pathology >Serum alpha1-proteinase inhibitor concentrations in healthy dogs - method validation and determination of reference interval and intra-individual variation.
【24h】

Serum alpha1-proteinase inhibitor concentrations in healthy dogs - method validation and determination of reference interval and intra-individual variation.

机译：健康犬的血清α 1 蛋白酶抑​​制剂浓度-方法验证和参考区间和个体内部变异的确定。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: A chronic loss of canine alpha 1-proteinase inhibitor (c alpha 1-PI) into the gastrointestinal (GI) tract could change the systemic proteinase-proteinase inhibitor balance. Serum c alpha 1-PI concentrations have not been studied in dogs with well-defined GI diseases. Objectives: To further evaluate serum c alpha 1-PI concentrations in dogs with GI diseases, the objectives of this study were to (1) analytically validate a previously developed fecal c alpha 1-PI immunoassay to determine serum concentrations, (2) determine a population-based reference interval (RI) and assess the clinical utility, (3) determine stability of serum c alpha 1-PI, (4) determine the intra-individual variation in healthy dogs, and (5) determine the clinically relevant magnitude of change of serum c alpha 1-PI. Methods: Prestudy validation of the 125I-c alpha 1-PI immunoassay included linearity, spiking recovery, and intra- and inter-assay precision. A RI was calculated with samples of healthy dogs. Stability at -20 degrees C was tested on 36 samples. Intra-individual variation was assessed using samples collected from 11 healthy dogs over a 12-week period. Results: The c alpha 1-PI radioimmunoassay (RIA) was linear, accurate, precise, and reproducible. Serum c alpha 1-PI decreased by 11% after one year at -20 degrees C. Analytical, intra-individual, inter-individual, and total variation were 6.4, 9.9, 9.0, and 25.3%, respectively. The RI for serum c alpha 1-PI was 732-1802 mg/L (n=87); there were no differences between sex and age groups. The index of individuality was 1.31. As analytical variation was >1/2 inter-individual variation, the minimum critical difference was not determined. Conclusions: The results of this study provide the basis for further evaluating serum c alpha 1-PI in dogs with GI disease. Using a population-based RI for serum c alpha 1-PI appears to be appropriate.
机译：背景：犬α 1 -蛋白酶抑制剂（c alpha 1 -PI）长期流失到胃肠道可能会改变全身蛋白酶抑制剂的平衡。尚未对具有明确胃肠道疾病的犬进行血清c alpha 1 -PI浓度的研究。目的：为了进一步评估胃肠道疾病犬的血清c alpha 1 -PI浓度，本研究的目的是（1）分析验证先前开发的粪便c alpha 1 -PI免疫测定来确定血清浓度，（2）确定基于人群的参考间隔（RI）并评估临床效用，（3）确定血清c alpha 1 -PI的稳定性，（4 ）确定健康犬的个体内部差异，以及（5）确定血清c alpha 1 -PI变化的临床相关幅度。方法： 125 I-c alpha 1 -PI免疫测定的前期验证包括线性，加标回收率以及测定内和测定间精度。用健康狗的样品计算RI。测试了36个样品在-20摄氏度下的稳定性。在12周的时间内，使用从11只健康犬中收集的样本评估了个体内部差异。结果：c alpha 1 -PI放射免疫测定（RIA）线性，准确，精确且可重复。 -20°C一年后，血清c alpha 1 -PI下降了11％。分析性，个体内部，个体间和总变化为6.4％，9.9％，9.0％和25.3％，分别。血清c alpha 1 -PI的RI为732-1802 mg / L（n = 87）;性别和年龄组之间没有差异。个性指数为1.31。由于分析变异> 1/2个体间变异，因此未确定最小临界差。结论：本研究结果为进一步评估胃肠道疾病犬的血清cα 1 -PI提供了依据。对血清c alpha 1 -PI使用基于人群的RI似乎是合适的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号