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首页> 外文期刊>Veterinary Dermatology >Characterization and quantification of ceramides in the nonlesional skin of canine patients with atopic dermatitis compared with controls
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Characterization and quantification of ceramides in the nonlesional skin of canine patients with atopic dermatitis compared with controls

机译：与对照组相比，特应性皮炎犬科动物非病变皮肤中神经酰胺的表征和定量

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摘要

As in humans, there is mounting evidence in support of an abnormal skin barrier contributing to the pathogenesis of canine atopic dermatitis (AD). Studies in people with AD have associated an abnormal skin barrier with deficiencies in ceramides, which represent important components of the stratum corneum (SC) intercellular lipid lamellae. Therefore, the goal of this study was to determine if the SC of dogs with AD is deficient in ceramides compared to normal dogs. Samples of SC were obtained from nonlesional skin of the caudal abdomen of 14 patients with AD and 14 age-, breed- and sex-matched healthy controls using a cyanoacrylate stripping procedure, and the subclass and relative amount of ceramides were assessed blindly by thin layer chromatography. Paired t-tests using R statistical computer software revealed the percentage amounts of ceramides 1 and 9 were significantly lower in nonlesional skin of AD dogs compared to controls (P= 0.034 and P= 0.047, respectively), and the cholesterol percentage amount was significantly higher in AD dogs than in controls (P= 0.016). Furthermore, the cholesterol/ceramide ratio was significantly higher in the AD group with respect to controls (P= 0.014). These findings suggest that decreased amounts of ceramides in the skin of dogs with AD may be involved in the impaired barrier function of their skin.
机译：与人类一样，越来越多的证据表明异常的皮肤屏障有助于犬特应性皮炎（AD）的发病。患有AD的人的研究将异常的皮肤屏障与神经酰胺的缺乏联系在一起，神经酰胺的缺乏代表了角质层（SC）细胞间脂质薄片的重要成分。因此，本研究的目的是确定与正常犬相比，患有AD的犬的SC是否缺乏神经酰胺。使用氰基丙烯酸酯剥离法从14例AD患者和14例年龄，品种和性别匹配的健康对照的尾腹部非病变皮肤中获得SC样品，并通过薄层盲目评估神经酰胺的亚类和相对量层析。使用R统计计算机软件进行的配对t检验显示，与对照组相比，AD犬非病变皮肤中神经酰胺1和9的百分比含量显着降低（分别为P = 0.034和P = 0.047），并且胆固醇的含量明显更高在AD犬中比在对照组中（P ＝ 0.016）。此外，AD组的胆固醇/神经酰胺比率相对于对照组显着更高（P = 0.014）。这些发现表明，患有AD的狗的皮肤中神经酰胺含量的降低可能与它们的皮肤屏障功能受损有关。

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