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首页> 外文期刊>Veterinary Microbiology >Effect of porcine circovirus type 2a or 2b on infection kinetics and pathogenicity of two genetically divergent strains of porcine reproductive and respiratory syndrome virus in the conventional pig model.
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Effect of porcine circovirus type 2a or 2b on infection kinetics and pathogenicity of two genetically divergent strains of porcine reproductive and respiratory syndrome virus in the conventional pig model.

机译：2a或2b型猪圆环病毒对常规猪模型中两种遗传分化的猪繁殖与呼吸综合征病毒基因株的感染动力学和致病性的影响。

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摘要

To determine differences in infection kinetics of two temporally and genetically different type 2 porcine reproductive and respiratory syndrome virus (PRRSV) isolates in vivo with and without concurrent porcine circovirus (PCV) type 2a or 2b infection, 62 pigs were randomly assigned to one of seven groups: negative controls (n=8); pigs coinfected with a 1992 PRRSV strain (VR-2385) and PCV2a (CoI-92-2a; n=9), pigs coinfected with VR-2385 and PCV2b (CoI-92-2b; n=9), pigs coinfected with a 2006 PRRSV strain (NC16845b) and PCV2a (CoI-06-2a; n=9), pigs coinfected with NC16845b and PCV2b (CoI-06-2b; n=9), pigs infected with VR-2385 (n=9), and pigs infected with NC16845b (n=9). Blood samples were collected before inoculation and at day post-inoculation (dpi) 3, 6, 9 and 12 and tested for the presence of PRRSV antibody and RNA, PCV2 antibody and DNA, complete blood counts, and interferon gamma (IFN-gamma) levels. Regardless of concurrent PCV2 infection, VR-2385 initially replicated at higher levels and reached peak replication levels at dpi 6. Pigs infected with VR-2385 had significantly higher amounts of viral RNA in serum on both dpi 3 and dpi 6, compared to pigs infected with NC16845b. The peak of NC16845b virus replication occurred between dpi 9 and dpi 12 and was associated with a delayed anti-PRRSV antibody response in these pigs. PCV2 coinfection resulted in significantly more severe macroscopic and microscopic lung lesions and a stronger anti-PRRSV IgG response compared to pigs infected with PRRSV alone. This work further emphasizes in vivo replication differences among PRRSV strains and the importance of coinfecting pathogens. Copyright Copyright 2012 Elsevier B.V. All rights reserved.
机译：为了确定两种在时间上和遗传上不同的2型猪繁殖与呼吸综合征病毒（PRRSV）分离株在体内是否同时感染2a或2b型猪圆环病毒（PCV）的感染动力学差异，将62头猪随机分配到7头中的一只组：阴性对照（n = 8）;共同感染了1992 PRRSV株（VR-2385）和PCV2a（CoI-92-2a; n = 9）的猪，共同感染了VR-2385和PCV2b（CoI-92-2b; n = 9）的猪，共同感染了PRV的猪2006 PRRSV株（NC16845b）和PCV2a（CoI-06-2a; n = 9），同时感染NC16845b和PCV2b（CoI-06-2b; n = 9）的猪，受VR-2385感染的猪（n = 9），和感染了NC16845b的猪（n = 9）。在接种前和接种后第3、6、9和12天（dpi）采集血样，并测试PRRSV抗体和RNA，PCV2抗体和DNA的存在，全血细胞计数和干扰素γ（IFN-γ）水平。无论并发PCV2感染如何，VR-2385最初都会以更高的水平复制并在dpi 6达到峰值复制水平。与感染的猪相比，感染VR-2385的猪在dpi 3和dpi 6时血清中的病毒RNA量明显更高使用NC16845b。 NC16845b病毒复制的峰值出现在dpi 9和dpi 12之间，并且与这些猪的抗PRRSV抗体反应延迟有关。与单独感染PRRSV的猪相比，PCV2合并感染导致明显更严重的宏观和微观肺部病变以及更强的抗PRRSV IgG反应。这项工作进一步强调了PRRSV病毒株之间的体内复制差异以及共感染病原体的重要性。版权版权所有2012 Elsevier B.V.保留所有权利。

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