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首页> 外文期刊>Veterinary Microbiology >Experimental infection and comparative genomic analysis of a highly pathogenic PRRSV-HBR strain at different passage levels.
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Experimental infection and comparative genomic analysis of a highly pathogenic PRRSV-HBR strain at different passage levels.

机译：高致病性PRRSV-HBR株在不同传代水平下的实验感染和比较基因组分析。

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摘要

A highly pathogenic strain of porcine reproductive and respiratory syndrome virus (PRRSV-HBR) was passaged on Marc-145 cells for 125 passages. In order to elucidate the change in virulence of PRRSV-HBR strain during the process of passage in vitro, swine infection experiment was performed with the viruses of low (F5 and F10) and high passage (F125). In addition, to identify the mutations related to the change in virulence of PRRSV-HBR strain, we compared and analyzed the genomic sequences of the F5, F10 and F125 of the strain. The virulence of F125 was significantly lower than that of F5 in the virus-infected pigs. In comparison with F5 and F125, there were 45 amino acids (aa) mutations and a deletion of 2 continuous aa by means of the virus genome sequence analysis. For these mutations, 33 aa (73.3%) occurred in the viral nonstructural proteins and the other 12 aa (26.7%) were contained in the viral structural proteins. Of the mutations, only 15 aa (33.3%) appeared in F10 and 30 aa (66.7%) occurred during passage from F10 to F125. The data showed that the latter 30 aa mutations were probably associated with attenuation of PRRSV-HBR strain, and that the change in virulence of the virus was determined by multiple alterations both in the structural and nonstructural genes. The virulence of PRRSV-HBR strain was remarkably attenuated after serial passages, and it can be used as vaccine candidate for control of the PRRS.
机译：一株高致病性猪繁殖与呼吸综合征病毒株（PRRSV-HBR）在Marc-145细胞上传代125次。为了阐明PRRSV-HBR毒株在体外传代过程中的毒力变化，对低（F5和F10）和高通过（F125）病毒进行了猪感染实验。另外，为了鉴定与PRRSV-HBR菌株的毒力变化相关的突变，我们比较并分析了该菌株的F5，F10和F125的基因组序列。在病毒感染的猪中，F125的毒力明显低于F5。与F5和F125相比，通过病毒基因组序列分析，有45个氨基酸（aa）突变和2个连续氨基酸的缺失。对于这些突变，病毒非结构蛋白中发生了33个氨基酸（73.3％），而病毒结构蛋白中包含了其他12个氨基酸（26.7％）。在这些突变中，只有10个氨基酸（33.3％）出现在F10中，而30个氨基酸（66.7％）发生在从F10到F125的过程中。数据表明，后30个氨基酸突变可能与PRRSV-HBR株的减毒有关，并且病毒的毒力变化是由结构和非结构基因的多次改变决定的。连续传代后，PRRSV-HBR毒株的毒力显着减弱，可作为控制PRRS的候选疫苗。

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