• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Veterinary Ophthalmology >Canine pre-iridal fibrovascular membranes: morphologic and immunohistochemical investigations
【24h】

Canine pre-iridal fibrovascular membranes: morphologic and immunohistochemical investigations

机译：犬虹膜前纤维血管膜：形态学和免疫组化研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Pathologic intraocular neovascularization is a key component of many canine ophthalmic diseases such as uveitis, retinal detachment, intraocular neoplasms, and corneal perforation. The purpose of this study was to evaluate the structure of pre-iridal fibrovascular membranes (PIFMs) associated with several different disease processes and to identify specific factors associated with their development in the canine eye. This study examined 36 enucleated canine eyes with the diagnosis of PIFM and one of the following: lens-induced uveitis, retinal detachment, iridociliary adenoma, corneal perforation, severe hyphema, or vitreal gliovascular membranes (canine ocular gliovascular syndrome, COGS). Three histologic stains and six immunohistochemical stains were performed in all 36 PIFM eyes and four histologically normal eyes, including: hematoxylin and eosin, alcian blue periodic acid schiff (PAS), Masson's trichrome, platelet endothelial cell adhesion molecule-1 (CD31), smooth muscle actin, vimentin, laminin, vascular endothelial growth factor (VEGF), and cyclooxygenase-2 (COX-2). Pre-iridal fibrovascular membrane extracellular matrix staining was consistent with collagen and mucins in all cases and positive for laminin in most cases. All PIFMs contained CD31-positive vessels and predominantly lymphoplasmacytic inflammation. Both PIFM vessels and spindle cells were positive for laminin, vimentin, smooth muscle actin, VEGF, and COX-2. Secondary intraocular pathology and immunohistochemical staining of other intraocular structures are also reported. Pre-iridal fibrovascular membrane morphology and immunohistochemical characteristics were similar across six canine disease processes, suggesting analogous pathophysiologic mechanisms. COX-2 and VEGF were identified using immunohistochemistry and may play a role in PIFM development.
机译：病理性眼内新血管形成是许多犬眼病的关键组成部分，如葡萄膜炎，视网膜脱离，眼内肿瘤和角膜穿孔。这项研究的目的是评估与几种不同疾病过程相关的虹膜前纤维血管膜（PIFM）的结构，并确定与其在犬眼中发育相关的特定因素。这项研究检查了36例无晶状体的犬眼，并诊断为PIFM和以下之一：晶状体引起的葡萄膜炎，视网膜脱离，虹膜睫状腺瘤，角膜穿孔，严重的前房血或玻璃体胶质血管膜（犬眼胶质血管综合征，COGS）。在所有36只PIFM眼和4例组织学正常的眼睛中进行了3个组织学染色和6个免疫组织化学染色，包括：苏木精和曙红，阿辛蓝高碘酸席夫（PAS），马森三色，血小板内皮细胞粘附分子-1（CD31）肌动蛋白，波形蛋白，层粘连蛋白，血管内皮生长因子（VEGF）和环氧合酶2（COX-2）。虹膜前纤维血管膜细胞外基质染色在所有情况下均与胶原蛋白和粘蛋白一致，在大多数情况下为层粘连蛋白阳性。所有PIFM都包含CD31阳性血管，并且主要是淋巴浆细胞性炎症。 PIFM血管和纺锤状细胞均对层粘连蛋白，波形蛋白，平滑肌肌动蛋白，VEGF和COX-2呈阳性。还报道了其他眼内结构的继发性眼内病理学和免疫组织化学染色。在六个犬病过程中，虹膜前纤维血管膜的形态和免疫组织化学特征相似，表明相似的病理生理机制。使用免疫组织化学鉴定了COX-2和VEGF，它们可能在PIFM的发展中起作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号