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首页> 外文期刊>Virus Genes >Divergent molecular variants of Grapevine virus B (GVB) from corky bark (CB)-affected and CB-negative LN33 hybrid grapevines.
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Divergent molecular variants of Grapevine virus B (GVB) from corky bark (CB)-affected and CB-negative LN33 hybrid grapevines.

机译：受软木树皮（CB）和CB阴性LN33杂交葡萄的葡萄病毒B（GVB）的不同分子变异。

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摘要

Analysis of two Grapevine virus B (GVB)-infected LN33 hybrid grapevines revealed that a plant exhibiting clear symptoms of corky bark (CB) disease was infected with two molecular variants of the virus, whereas a plant exhibiting no disease symptoms was infected with only one variant. Sequence results indicated that the single variant in the CB-negative grapevine was also one of the two present in the CB-affected hybrid. Plant extracts from these two grapevines were used to successfully transmit the virus to N. benthamiana. After further cloning and sequencing, two clearly divergent variants were identified. Comparative molecular analysis of the variants, named here GVB 953-1 and GVB-H1, respectively, transmitted from CB-affected and consistently CB-negative plants, revealed short genomic regions, most of them highly divergent, that encoded amino acid sequences, containing significant amino acid substitutions altering the net charges of their respective proteins. Interestingly, a comparison of these variants to genome sequence data of GVB variants GVB Italy and GVB 94/971 available from the GenBank, revealed that these significant amino acid substitutions were the same for, and unique to, the variant pairs GVB 953-1/GVB Italy and GVB-H1/GVB 94/971. This despite the variants of each pair being otherwise clearly different at nucleotide and amino acid levels. In addition, both sets of variants differed substantially in their respective 3'-non-translated (3'NTR) regions. The relevance of these findings is discussed.
机译：对两株感染了乙型肝炎病毒（GVB）的LN33杂种葡萄的分析表明，显示出明显树皮病（CB）症状的植物感染了该病毒的两种分子变体，而无病害的植物仅感染了一种。变体。序列结果表明，CB阴性葡萄树中的单个变异体也是受CB影响的杂种中存在的两个变异体之一。从这两个葡萄树中提取的植物被用来成功地将病毒传播给本氏烟草。进一步克隆和测序后，鉴定出两个明显不同的变体。分别从受CB影响和始终为CB阴性的植物传播的变体（分别命名为GVB 953-1和GVB-H1）的比较分子分析显示，基因组区域较短，其中大多数高度趋异，编码的氨基酸序列包含显着的氨基酸取代改变了它们各自蛋白质的净电荷。有趣的是，将这些变体与可从GenBank获得的GVB变体GVB Italy和GVB 94/971的基因组序列数据进行比较后发现，这些显着的氨基酸取代对于变体对GVB 953-1 /是相同的，并且是唯一的。意大利GVB和GVB-H1 / GVB 94/971。尽管每对变体在核苷酸和氨基酸水平上明显不同，但这是不可行的。另外，两组变体在它们各自的3'-非翻译（3'NTR）区域中基本上不同。讨论了这些发现的相关性。

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