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首页> 外文期刊>Virus Research: An International Journal of Molecular and Cellular Virology >Down-regulation of the cyprinid herpesvirus-3 annotated genes in cultured cells maintained at restrictive high temperature
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Down-regulation of the cyprinid herpesvirus-3 annotated genes in cultured cells maintained at restrictive high temperature

机译：维持在限制性高温下的培养细胞中塞浦路斯疱疹病毒3注释基因的下调

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摘要

Cyprinid herpesvirus-3 (CyHV-3) is a member of the Alloherpesviridae, in the order Herpesvirales. It causes a fatal disease in carp and koi fish. The disease is seasonal and is active when water temperatures ranges from 18 to 28 °C. Little is known about how and where the virus is preserved between the permissive seasons. The hallmark of the herpesviruses is their ability to become latent, persisting in the host in an apparently inactive state for varying periods of time. Hence, it could be expected that CyHV-3 enter a latent period. CyHV-3 has so far been shown to persist in fish maintained under restrictive temperatures, while shifting the fish to permissive conditions reactivates the virus. Previously, we demonstrated that cultured cells infected with CyHV-3 at 22 °C and subsequently transferred to a restrictive temperature of 30 °C preserve the virus for 30 days. The present report shows that cultured carp cells maintained and exposed to CyHV-3 at 30 °C are abortively infected; that is, autonomous viral DNA synthesis is hampered and the viral genome is not multiplied. Under these conditions, 91 of the 156 viral annotated ORFs were initially transcribed. These transcripts were down-regulated and gradually shut off over 18 days post-infection, while two viral transcripts encoded by ORFs 114 and 115 were preserved in the infected cells for 18 days p.i. These experiments, carried out in cultured cells, suggest that fish could be infected at a high non-permissive temperature and harbor the viral genome without producing viral particles.
机译：塞浦路斯疱疹病毒3（CyHV-3）是Alloherpesviridae的成员，顺序为疱疹病毒。它在鲤鱼和锦鲤鱼中引起致命的疾病。该疾病是季节性的，在水温为18至28°C时活跃。关于在许可的季节之间如何以及在何处保存病毒知之甚少。疱疹病毒的标志是它们具有潜伏的能力，可以以明显处于非活动状态的状态在宿主中持续不同的时间。因此，可以预期CyHV-3进入潜伏期。迄今为止，CyHV-3被证明可以在限制温度下维持在鱼类中，而将鱼类转移到允许的条件下可以重新激活该病毒。以前，我们证明了在22°C感染CyHV-3并随后转移至30°C限制性温度的培养细胞可将病毒保存30天。本报告显示，维持并暴露于30°C的CyHV-3的养殖鲤鱼细胞被流产感染;也就是说，自主病毒DNA的合成受到阻碍，病毒基因组没有倍增。在这些条件下，最初转录了156个带病毒注释的ORF中的91个。这些转录本被下调并在感染后18天内逐渐关闭，而由ORF 114和115编码的两个病毒转录本在感染的细胞中被保存了18天。这些在培养细胞中进行的实验表明，鱼类可能会在较高的非许可温度下被感染，并携带病毒基因组而不会产生病毒颗粒。

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