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首页> 外文期刊>Virus Research: An International Journal of Molecular and Cellular Virology >Comparative analysis of virus-specific small RNA profiles of three biologically distinct strains of Potato virus Y in infected potato (Solarium tuberosum) cv. Russet Burbank
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Comparative analysis of virus-specific small RNA profiles of three biologically distinct strains of Potato virus Y in infected potato (Solarium tuberosum) cv. Russet Burbank

机译：三种受感染的马铃薯马铃薯马铃薯Y病毒在生物学上不同的菌株的病毒特异性小RNA谱的比较分析。拉斯塞特·伯班克

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摘要

Deep sequencing technology has enabled the analysis of small RNA profiles of virus-infected plants and could provide insights into virus-host interactions. Potato virus Y is an economically important viral pathogen of potato worldwide. In this study, we investigated the nature and relative levels of virus-derived small interfering RNAs (vsiRNAs) in potato cv. Russet Burbank infected with three biologically distinct and economically important strains of PVY, the ordinary strain (PVY-O), tobacco veinal-necrotic strain (PVY-N) and tuber necrotic strain (PVY-NTN). The analysis showed an overall abundance of vsiRNAs of 20-24 nt in PVY-infected plants. Considerable differences were present in the distribution of vsiRNAs as well as total small RNAs. The 21 nt class was the most prevalent in PVY-infected plants irrespective of the virus strain, whereas in healthy potato plants, the 24 nt class was the most dominant. vsiRNAs were derived from every position in the PVY genome, though certain hotspots were identified for each of the PVY strains. Among the three strains used, the population of vsiRNAs of different size classes was relatively different with PVY-NTN accumulating the highest level of vsiRNAs, while PVY-N infected plants had the least population of vsiRNAs. Unique vsiRNAs mapping to PVY genome in PVY-infected plants amounted to 3.13,1.93 and 1.70% for NTN, N and 0, respectively. There was a bias in the generation of vsiRNAs from the plus strand of the genome in comparison to the negative strand. The highest number of total vsiRNAs was from the cytoplasmic inclusion protein gene (CI) in PVY-0 and PVY-NTN strains, whereas from PVY-N, the Nib gene produced maximum total vsiRNAs. These findings indicate that the three PVY strains interact differently in the same host genetic background and provided insights into virus-host interactions in an important food crop.
机译：深度测序技术已使分析受病毒感染的植物的小RNA谱成为可能，并且可以提供有关病毒与宿主相互作用的信息。马铃薯病毒Y是全世界马铃薯的重要经济病毒病原体。在这项研究中，我们调查了马铃薯cv中病毒衍生的小干扰RNA（vsiRNA）的性质和相对水平。 Russet Burbank感染了三种在生物学上和经济上重要的PVY菌株：普通菌株（PVY-O），烟草静脉坏死菌株（PVY-N）和块茎坏死菌株（PVY-NTN）。分析显示在PVY感染的植物中vsiRNA的总体丰度为20-24nt。 vsiRNA的分布以及小RNA的总含量存在很大差异。不论病毒株如何，21 nt类在PVY感染的植物中最普遍，而在健康马铃薯植株中，24 nt类是最主要的。 vsiRNAs来自PVY基因组中的每个位置，尽管对于每个PVY菌株都鉴定出某些热点。在使用的三种菌株中，不同大小类别的vsiRNA的群体相对不同，PVY-NTN积累了最高水平的vsiRNA，而被PVY-N感染的植物的vsiRNA群体最少。 NTN，N和0分别映射到PVY感染植物中PVY基因组的独特vsiRNA分别为3.13％，1.93％和1.70％。与负链相比，从基因组正链产生的vsiRNA存在偏差。 vsiRNA总数最多的是来自PVY-0和PVY-NTN菌株的胞质包涵蛋白基因（CI），而Nis基因产生的PViRNA数量最多，来自PVY-N。这些发现表明，三种PVY菌株在同一宿主遗传背景下相互作用不同，并为重要粮食作物中的病毒-宿主相互作用提供了见识。

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